LOCUS       sequence-142920-         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mKate2 (codon adapted for Streptococcus pneumoniae); under
            control of CSP inducible promoter PssbB. Plasmid integrates in S. 
            pneumoniae bgaA locus by double cross-over..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Beilharz K, van Raaphorst R, Kjos M, Veening JW
  TITLE     Red fluorescent proteins for gene expression and protein 
            localization studies in Streptococcus pneumoniae and efficient 
            transformation with Gibson assembled DNA.
  JOURNAL   Appl Environ Microbiol. 2015 Aug 7. pii: AEM.02033-15.
  PUBMED    26253684
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2015 Aug 7. pii: AEM.02033-15."
COMMENT     Sequence Label: pKB01-PssbB-mKate2
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tgcgacggct acttcgtgct gttcaacata agtttcctta tctgcttctt tgatacgttc
       61 taaacgacga tcgacgtagt aaacaccagg catcttaaga ttcttagctg gctttttaga
      121 acggtatgtg gtcttcaaat tacagataag gtgacctcca ccgacaagtt tcaatgccat
      181 atcagcacga ccttccaatc caccatcggc aggatataat gtttcggtag atgcttccca
      241 tccaagggtc tttttctgca taacaggacc attacttgga aaattaactc cacggatctt
      301 tacattgtag ataagacaac catcttgcaa agatgtatct tgagttgctg tcaatactcc
      361 accatcttca taagttgtga cacgttccca tgtaaatcct tcaggaaatg actgtttaaa
      421 gaaatctggg ataccttggg tatgattgat aaaagtcttt gatccgtaca taaaagatgt
      481 agcaagaata tcaaaggcaa aaggcaatgg tccaccttca acggccttaa tacgcatggt
      541 ttgagtacct tcatatggtt ttccttctcc ttctgaggta catttaaagt ggtgattatt
      601 tactgttcct tccatgtaca atttcatgtg catattttcc ttgataagtt ctgacattaa
      661 ttttcctcct ctagaatatg atatcaatgg atccggtgta gacgttaaac gcccaatcat
      721 gataacttta ttatacatat tttcttcctc ctacttatct attcgtagga aatcaaaaaa
      781 agttacagaa atttgtaact tttcgaggaa atttcttatt ttt
//
