LOCUS       sequence-142922-         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mKate2 (codon adapted for Streptococcus pneumoniae); under
            control of CSP inducible promoter PssbB. Plasmid integrates in S. 
            pneumoniae bgaA locus by double cross-over..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Beilharz K, van Raaphorst R, Kjos M, Veening JW
  TITLE     Red fluorescent proteins for gene expression and protein 
            localization studies in Streptococcus pneumoniae and efficient 
            transformation with Gibson assembled DNA.
  JOURNAL   Appl Environ Microbiol. 2015 Aug 7. pii: AEM.02033-15.
  PUBMED    26253684
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2015 Aug 7. pii: AEM.02033-15."
COMMENT     Sequence Label: pKB01-PssbB-mKate2
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cgaaacgcgc gaggcaggct aaacctgctg ctactgctgc ttggcttgcc gcactacctg
       61 tatttggtaa ctgggcttta ttagtttgat cagcttcact tgaatcaatt ggttttgtat
      121 ctgctttttc tgacacttgt ggttttttag cttcttgagc tactggtttg gttccaacca
      181 agacgatgcg gtctgtcgga acttctacca cttcacggag tttttcttcc ttacttccat
      241 caggattaat cactgtaaag atacgttctt ttccaacttt tccttcttgt tctacacgag
      301 tttcacctag atacagtgtt gaatcttttt tctcaactgt cttgtaggcc aaatcttttt
      361 caacaaattc gagttttgga agatcttctt gtacagcaga aactggtttt tccttagtca
      421 actggatacg gtattccttg acttgttttc cactttctga aacgaggcga acaagtactg
      481 gaaggctatc ttctccacta tctaccacag ttgaagctac ttgattgttt tcttcaactg
      541 agacttttgg ccgttgacct ttataggtaa tttgatagtc ttgacgattt tcagcgaaat
      601 cagcaagttc ttttccatct acaagaatct ttgattgagt gctttcttga ggcaattcac
      661 ttggtgcaag gaaggtcatc tcaatcatcg caacaccgct cttgtcagct ttacgctcca
      721 tacgccatct catgactttg gctttgacag ctttaaatgt tacgttgatt tcatcaccag
      781 aaatagtaca taatggattt ccttacgcga aatacgggca gacatggcct gcccggttat
      841 tattattttt gacaccagac caactggtaa tggtagcgac cggcgctcag cgatagcgct
      901 atcaaaagaa tcttgcttgg caaggttctt tttt
//
