LOCUS       sequence-142929-        1023 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SNAP29-V5 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Schaffer BE, Levin RS, Hertz NT, Maures TJ, Schoof ML, Hollstein PE,
            Benayoun BA, Banko MR, Shaw RJ, Shokat KM, Brunet A
  TITLE     Identification of AMPK Phosphorylation Sites Reveals a Network of 
            Proteins Involved in Cell Invasion and Facilitates Large-Scale 
            Substrate Prediction.
  JOURNAL   Cell Metab. 2015 Nov 3;22(5):907-21. doi: 
            10.1016/j.cmet.2015.09.009. Epub 2015 Oct 8.
  PUBMED    26456332
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2015.09.009"; journalName: "Cell Metab"; date: 
            "2015-11-3- 3"; volume: "22"; issue: "5"; pages: "907-21"
COMMENT     Sequence Label: pcDNA 3.2/V5-DEST SNAP29-V5
FEATURES             Location/Qualifiers
     source          1..1023
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    100..124
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(899..923)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             949..990
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 cagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag ctggctagtt aagctatcaa caagtttgta caaaaaagtt
      121 ggcatgtcag cttaccctaa gagctacaat ccgttcgacg acgacgggga ggacgaaggc
      181 gcccggccgg ccccttggag ggacgcccga gacctccccg acgggcccga cgcgcccgcg
      241 gacaggcagc agtacttgcg gcaggaggtc ctccgcaggg ctgaggccac ggccgccagc
      301 accagcaggt ccctggccct catgtacgag tccgagaagg ttggggtcgc ctcttccgag
      361 gagctcgccc gtcagcgagg agtcctggag cgcacagaga agatggtgga caagatggac
      421 caagatttga agatcagcca gaaacacatc aatagcatta agagcgtgtt tggggggctg
      481 gtcaattact tcaaatccaa accagtagag accccacctg aacagaatgg caccctcacc
      541 tcccagccca acaacagatt gaaagaagct ataagtacaa gtaaagaaca ggaagcaaag
      601 taccaggcca gccacccaaa ccttagaaag ctggatgata cagaccctgt ccccagaggg
      661 gctggttctg ccatgagtac tgatgcttac ccaaagaacc cacaccttcg agcctatcac
      721 cagaagatcg acagcaacct agatgagctg tccatgggac tgggtcgtct gaaggacata
      781 gccctgggga tgcagacaga aattgaggag caagatgaca ttcttgaccg gctgacaacc
      841 aaagtggaca agttagatgt caacataaaa agcacagaaa gaaaagttcg acaactctgc
      901 ccaactttct tgtacaaagt ggttgatcta gagggcccgc ggttcgaagg taagcctatc
      961 cctaaccctc tcctcggtct cgattctacg cgtaccggtt agtaatgagt ttaaacgggg
     1021 gag
//
