LOCUS       sequence-142930-         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SNAP29-V5 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Schaffer BE, Levin RS, Hertz NT, Maures TJ, Schoof ML, Hollstein PE,
            Benayoun BA, Banko MR, Shaw RJ, Shokat KM, Brunet A
  TITLE     Identification of AMPK Phosphorylation Sites Reveals a Network of 
            Proteins Involved in Cell Invasion and Facilitates Large-Scale 
            Substrate Prediction.
  JOURNAL   Cell Metab. 2015 Nov 3;22(5):907-21. doi: 
            10.1016/j.cmet.2015.09.009. Epub 2015 Oct 8.
  PUBMED    26456332
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2015.09.009"; journalName: "Cell Metab"; date: 
            "2015-11-3- 3"; volume: "22"; issue: "5"; pages: "907-21"
COMMENT     Sequence Label: pcDNA 3.2/V5-DEST SNAP29-V5
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    176..224
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     249..268
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(308..349)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    375..399
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cgcagatctg ctatggcagg gcctgccgcc ccgacgttgg ctgcgagccc tgggccttca
       61 cccgaacttg gggggtgggg tggggaaaag gaagaaacgc gggcgtattg gccccaatgg
      121 ggtctcggtg gggtatcgac agagtgccag ccctgggacc gaaccccgcg tttatgaaca
      181 aacgacccaa cacccgtgcg ttttattctg tctttttatt gccgtcatag cgcgggttcc
      241 ttccggtatt gtctccttcc gtgtttcagt tagcctcccc cgtttaaact cattactaac
      301 cggtacgcgt agaatcgaga ccgaggagag ggttagggat aggcttacct tcgaaccgcg
      361 ggccctctag atcaaccact ttgtacaaga aagttgggca gagttgtcga acttttcttt
      421 ctgtgctttt tatgttgaca tctaacttgt ccactttggt tgtcagccgg tcaagaatgt
      481 catcttgctc ctcaatttct gtctgcatcc ccagggctat gtccttcaga cgacccagtc
      541 ccatggacag ctcatctagg ttgctgtcga tcttctggtg ataggctcga aggtgtgggt
      601 tctttgggta agcatcagta ctcatggcag aaccagcccc tctggggaca gggtctgtat
      661 catccagctt tctaaggttt gggtggctgg cctggtactt tgcttcctgt tctttacttg
      721 tacttatagc ttctttcaat ctgttgttgg gctgggaggt gagggtgcca ttctgttcag
      781 gtggggtctc tactggtttg gatttgaagt aattgaccag ccccccaaac acgctcttaa
      841 tgctattgat gtgtttctgg ctgatcttca aatcttggtc catcttgtcc a
//
