LOCUS       sequence-142932-        6576 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of WDR5-FLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6576)
  AUTHORS   Kim JY, Banerjee T, Vinckevicius A, Luo Q, Parker JB, Baker MR, 
            Radhakrishnan I, Wei JJ, Barish GD, Chakravarti D
  TITLE     A role for WDR5 in integrating threonine 11 phosphorylation to 
            lysine 4 methylation on histone H3 during androgen signaling and in 
            prostate cancer.
  JOURNAL   Mol Cell. 2014 May 22;54(4):613-25. doi: 
            10.1016/j.molcel.2014.03.043. Epub 2014 May 1.
  PUBMED    24793694
REFERENCE   2  (bases 1 to 6576)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.03.043"; journalName: "Mol Cell"; date: 
            "2014-05-22- 22"; volume: "54"; issue: "4"; pages: "613-25"
COMMENT     Sequence Label: p3XFLAG-CMV-14-WDR5
FEATURES             Location/Qualifiers
     source          1..6576
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(34..53)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     244..265
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     528..550
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     542..559
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     543..559
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        720..1099
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1100..1303
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1254..1274
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2358..2423
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    2434..3056
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2633..2652)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(3080..3100)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3098..3395
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3449..4243
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3503..3522)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4113..4132
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(4772..5360)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4852..4871)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5531..6322,6323..6391))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6154..6173
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6392..6496)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      join(6523..6576,1..402)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
       61 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
      121 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
      181 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
      241 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
      301 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
      361 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttccattcgc cattcaggct
      421 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      481 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      541 ttgtaaaacg acggccagtg ccaagctgat ctatacattg aatcaatatt ggcaattagc
      601 catattagtc attggttata tagcataaat caatattggc tattggccat tgcatacgtt
      661 gtatctatat cataatatgt acatttatat tggctcatgt ccaatatgac cgccatgttg
      721 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
      781 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
      841 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      901 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      961 agtgtatcat atgccaagtc cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     1021 gcattatgcc cagtacatga ccttacggga ctttcctact tggcagtaca tctacgtatt
     1081 agtcatcgct attaccatgg tgatgcggtt ttggcagtac accaatgggc gtggatagcg
     1141 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     1201 gcaccaaaat caacgggact ttccaaaatg tcgtaataac cccgccccgt tgacgcaaat
     1261 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
     1321 gaattaagct tcggatggcg acggaggaga agaagcccga gaccgaggcc gccagagcac
     1381 agccaacccc ttcgtcatcc gccactcaga gcaagcctac acctgtgaag ccaaactatg
     1441 ctctaaagtt cacccttgct ggccacacca aagcagtgtc ctccgtgaaa ttcagcccga
     1501 atggagagtg gctggcaagt tcatctgctg ataaacttat taaaatttgg ggcgcgtatg
     1561 atgggaaatt tgagaaaacc atatctggtc acaagctggg aatatccgat gtagcctggt
     1621 cgtcagattc taaccttctt gtttctgcct cagatgacaa aaccttgaag atatgggacg
     1681 tgagctcggg caagtgtctg aaaaccctga agggacacag taattatgtc ttttgctgca
     1741 acttcaatcc ccagtccaac cttattgtct caggatcctt tgacgaaagc gtgaggatat
     1801 gggatgtgaa aacagggaag tgcctcaaga ctttgccagc tcactcggat ccagtctcgg
     1861 ccgttcattt taatcgtgat ggatccttga tagtttcaag tagctatgat ggtctctgtc
     1921 gcatctggga caccgcctcg ggccagtgcc tgaagacgct catcgatgac gacaaccccc
     1981 ccgtgtcttt tgtgaagttc tccccgaacg gcaaatacat cctggccgcc acgctggaca
     2041 acactctgaa gctctgggac tacagcaagg ggaagtgcct gaagacgtac actggccaca
     2101 agaatgagaa atactgcata tttgccaatt tctctgttac tggtgggaag tggattgtgt
     2161 ctggctcaga ggataacctt gtttacatct ggaaccttca gacgaaagag attgtacaga
     2221 aactacaagg ccacacagat gtcgtgatct caacagcttg tcacccaaca gaaaacatca
     2281 tcgcctctgc tgcgctagaa aatgacaaaa caattaaact gtggaagagt gactgcgcct
     2341 ctagaggatc ccgggctgac tacaaagacc atgacggtga ttataaagat catgacatcg
     2401 actacaagga tgacgatgac aagtagtgat cccgggtggc atccctgtga cccctcccca
     2461 gtgcctctcc tggccctgga agttgccact ccagtgccca ccagccttgt cctaataaaa
     2521 ttaagttgca tcattttgtc tgactaggtg tccttctata atattatggg gtggaggggg
     2581 gtggtatgga gcaaggggca agttgggaag acaacctgta gggcctgcgg ggtctattgg
     2641 gaaccaagct ggagtgcagt ggcacaatct tggctcactg caatctccgc ctcctgggtt
     2701 caagcgattc tcctgcctca gcctcccgag ttgttgggat tccaggcatg catgaccagg
     2761 ctcagctaat ttttgttttt ttggtagaga cggggtttca ccatattggc caggctggtc
     2821 tccaactcct aatctcaggt gatctaccca ccttggcctc ccaaattgct gggattacag
     2881 gcgtgaacca ctgctccctt ccctgtcctt ctgattttaa aataactata ccagcaggag
     2941 gacgtccaga cacagcatag gctacctggc catgcccaac cggtgggaca tttgagttgc
     3001 ttgcttggca ctgtcctctc atgcgttggg tccactcagt agatgcctgt tgaattgggt
     3061 acgcggccag cttggctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     3121 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     3181 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     3241 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     3301 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     3361 gagctattcc agaagtagtg aggaggcttt tttggaggaa ttgatcagct tgggatctga
     3421 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     3481 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     3541 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     3601 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     3661 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     3721 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     3781 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     3841 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     3901 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     3961 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     4021 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     4081 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     4141 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     4201 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     4261 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
     4321 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     4381 cgcggggatc tcatgctgga gttcttcgcc caccccgggc tcgatcccct cgcgagttgg
     4441 ttcagctgct gcctgaggct ggacgacctc gcggagttct accggcagtg caaatccgtc
     4501 ggcatccagg aaaccagcag cggctatccg cgcatccatg cccccgaact gcaggagtgg
     4561 ggaggcacga tggccgcttt ggtcgacccg gacgggacgc tcctgcgcct gatacagaac
     4621 gaattgcttg caggcatctc atgagtgtgt cttcccgttt tccgcctgag gtcactgcgt
     4681 ggatggagcg ctggcgcctg ctgcgcgacg gcgagctgct caccacccac tcgccaagct
     4741 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     4801 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     4861 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     4921 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     4981 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     5041 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     5101 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     5161 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     5221 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     5281 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     5341 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     5401 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     5461 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     5521 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     5581 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     5641 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     5701 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     5761 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     5821 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     5881 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     5941 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     6001 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     6061 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     6121 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     6181 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     6241 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     6301 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     6361 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     6421 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     6481 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg
     6541 cattaagcgc ggcgggtgtg gtggttacgc gcagcg
//
