LOCUS       sequence-142935-         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Non-targeting control shRNA cloned in retroviral vector pMLP 
            (MSCV-based vector expressing shRNA in a mir30 context)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Acevedo M, Vernier M, Mignacca L, Lessard F, Huot G, Moiseeva O, 
            Bourdeau V, Ferbeyre G
  TITLE     A CDK4/6-Dependent Epigenetic Mechanism Protects Cancer Cells from 
            PML-induced Senescence.
  JOURNAL   Cancer Res. 2016 Jun 1;76(11):3252-64. doi: 
            10.1158/0008-5472.CAN-15-2347. Epub 2016 Mar 29.
  PUBMED    27206849
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/0008-5472.CAN-15-2347"; journalName: "Cancer Res"; date: 
            "2016-06-1- 1"; volume: "76"; issue: "11"; pages: "3252-64"
COMMENT     Sequence Label: shNTC
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           64..184
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           212..226
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           254..380
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        394..894
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(429..452)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     826..845
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 gccctcactc cttctctagg cgccggaatt agatctctcg actagggata acagggtaat
       61 tgtttgaatg aggcttcagt actttacaga atcgttgcct gcacatcttg gaaacacttg
      121 ctgggattac ttcttcaggt taacccaaca gaaggctcga gaaggtatat gctgttgaca
      181 gtgagcgata gcgactaaac acatcaattt agtgaagcca cagatgtaaa ttgatgtgtt
      241 tagtcgctag tgcctactgc ctcggaattc aaggggctac tttaggagca attatcttgt
      301 ttactaaaac tgaatacctt gctatctctt tgatacattt ttacaaagct gaattaaaat
      361 ggtataaatt aaatcacttt tttcaattct accgggtagg ggaggcgctt ttcccaaggc
      421 agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct
      481 ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc
      541 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg
      601 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca
      661 gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct
      721 ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg
      781 ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc
      841 aaaagcgcac gtctgccgcg ctgttctcct cttccctcat ctccgggcct ttcgaccctg
      901 cagccccaag c
//
