LOCUS       sequence-142937-         648 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Non-targeting control shRNA cloned in retroviral vector pMLP 
            (MSCV-based vector expressing shRNA in a mir30 context)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Acevedo M, Vernier M, Mignacca L, Lessard F, Huot G, Moiseeva O, 
            Bourdeau V, Ferbeyre G
  TITLE     A CDK4/6-Dependent Epigenetic Mechanism Protects Cancer Cells from 
            PML-induced Senescence.
  JOURNAL   Cancer Res. 2016 Jun 1;76(11):3252-64. doi: 
            10.1158/0008-5472.CAN-15-2347. Epub 2016 Mar 29.
  PUBMED    27206849
REFERENCE   2  (bases 1 to 648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/0008-5472.CAN-15-2347"; journalName: "Cancer Res"; date: 
            "2016-06-1- 1"; volume: "76"; issue: "11"; pages: "3252-64"
COMMENT     Sequence Label: shNTC
FEATURES             Location/Qualifiers
     source          1..648
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           complement(11..137)
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(165..179)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(207..327)
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(383..399)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(415..437)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 agaattgaaa aaagtgattt aatttatacc attttaattc agctttgtaa aaatgtatca
       61 aagagatagc aaggtattca gttttagtaa acaagataat tgctcctaaa gtagcccctt
      121 gaattccgag gcagtaggca ctagcgacta aacacatcaa tttacatctg tggcttcact
      181 aaattgatgt gtttagtcgc tatcgctcac tgtcaacagc atataccttc tcgagccttc
      241 tgttgggtta acctgaagaa gtaatcccag caagtgtttc caagatgtgc aggcaacgat
      301 tctgtaaagt actgaagcct cattcaaaca attaccctgt tatccctagt cgagagatct
      361 aattccggcg cctagagaag gagtgagggc tggataaagg gaggatcgag gcggggtcga
      421 acgaggaggt tcaaggggga gagacggggc ggatggagga agaggaggcg gaggcttagg
      481 gtgtacaaag ggcttgaccc agggaggggg gtcaaaagcc aaggcttccc aggtcacgat
      541 gtaggggacc tggtctgggt gtccatgcgg gccaggtgaa aagaccttga tcttaacctg
      601 ggtgatgagg tctcggttaa aggtgccgtc tcgcggccat ccgacgtt
//
