LOCUS       sequence-14302-e        1428 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1428)
  TITLE     TALE Nuclease (TALEN)
REFERENCE   2  (bases 1 to 1428)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: TALEN Backbone
FEATURES             Location/Qualifiers
     source          1..1428
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1..10
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10..75
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             82..102
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             832..1419
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 gccaccatgg actataagga ccacgacgga gactacaagg atcatgatat tgattacaaa
       61 gacgatgacg ataagatggc cccaaagaag aagcggaagg tcggtatcca cggagtccca
      121 gcagccgtag atttgagaac tttgggatat tcacagcagc agcaggaaaa gatcaagccc
      181 aaagtgaggt cgacagtcgc gcagcatcac gaagcgctgg tgggtcatgg gtttacacat
      241 gcccacatcg tagccttgtc gcagcaccct gcagcccttg gcacggtcgc cgtcaagtac
      301 caggacatga ttgcggcgtt gccggaagcc acacatgagg cgatcgtcgg tgtggggaaa
      361 cagtggagcg gagcccgagc gcttgaggcc ctgttgacgg tcgcgggaga gctgagaggg
      421 cctccccttc agctggacac gggccagttg ctgaagatcg cgaagcgggg aggagtcacg
      481 gcggtcgagg cggtgcacgc gtggcgcaat gcgctcacgg gagcacccct caacctgacc
      541 gagaccgtac atgaaacgca tggcacgggg tctcaactca cgcctgagca ggtagtggct
      601 attgcatcca attccggggg cagacccgca ctggagtcaa tcgtggccca gctttcgagg
      661 ccggaccccg cgctggccgc actcactaat gatcatcttg tagcgctggc ctgcctcggc
      721 ggacgacccg ccttggatgc ggtgaagaag gggctcccgc acgcgcctgc attgattaag
      781 cggaccaaca gaaggattcc cgagaggaca tcacatcgag tggcaggttc ccaactcgtg
      841 aagagtgaac ttgaggagaa aaagtcggag ctgcggcaca aattgaaata cgtaccgcat
      901 gaatacatcg aacttatcga aattgctagg aactcgactc aagacagaat ccttgagatg
      961 aaggtaatgg agttctttat gaaggtttat ggataccgag ggaagcatct cggtggatca
     1021 cgaaaacccg acggagcaat ctatacggtg gggagcccga ttgattacgg agtgatcgtc
     1081 gacacgaaag cctacagcgg tgggtacaat cttcccatcg ggcaggcaga tgagatgcaa
     1141 cgttatgtcg aagaaaatca gaccaggaac aaacacatca atccaaatga gtggtggaaa
     1201 gtgtatcctt catcagtgac cgagtttaag tttttgtttg tctctgggca tttcaaaggc
     1261 aactataagg cccagctcac acggttgaat cacattacga actgcaatgg tgcggttttg
     1321 tccgtagagg aactgctcat tggtggagaa atgatcaaag cgggaactct gacactggaa
     1381 gaagtcagac gcaagtttaa caatggcgag atcaatttcc gctcataa
//
