LOCUS       sequence-14384-e        7735 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7735)
  AUTHORS   Tabor JJ, Levskaya A, Voigt CA
  TITLE     Multichromatic control of gene expression in Escherichia coli.
  JOURNAL   J Mol Biol. 2011 Jan 14. 405(2):315-24.
  PUBMED    21035461
REFERENCE   2  (bases 1 to 7735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2011 Jan 14. 405(2):315-24."
COMMENT     Sequence Label: pJT106b3
FEATURES             Location/Qualifiers
     source          1..7735
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             146..3190
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGSLSQMWIGDKNQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQK"
     primer_bind     complement(148..170)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      3225..3296
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3312..3339
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             3487..4197
                     /codon_start=1
                     /gene="cI"
                     /product="phage lambda repressor"
                     /label=lambda repressor
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
     CDS             4198..4230
                     /codon_start=1
                     /product="C-terminal peptide that mediates degradation in 
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
                     /label=ssrA tag (LVA)
                     /note="mutant LVA variant that confers accelerated 
                     degradation under some conditions (Andersen et al., 1998)"
                     /translation="AANDENYALVA"
     terminator      4278..4349
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4365..4392
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    4399..4419
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      4420..4477
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(4501..4518)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(4780..5730)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      5778..6000
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(6492..6586)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(6616..7407,7408..7476))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7239..7258
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7477..7581)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7646..7664
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(7668..7711)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    7714..7735
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 taagcacctg taggatcgta caggttgaca acaagaaaat ggtgtgttat agtcgaataa
       61 caccgtgcgt gttgactatt ttacctctgg cggtgataac acactgttcc ccaggacatc
      121 ctaacgccgc gccatgatag atcccgtcgt tttacaacgt cgtgactggg aaaaccctgg
      181 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
      241 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgctt
      301 tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg ctggagtgcg atcttcctga
      361 ggccgatact gtcgtcgtcc cctcaaactg gcagatgcac ggttacgatg cgcccatcta
      421 caccaacgtg acctatccca ttacggtcaa tccgccgttt gttcccacgg agaatccgac
      481 gggttgttac tcgctcacat ttaatgttga tgaaagctgg ctacaggaag gccagacgcg
      541 aattattttt gatggcgtta actcggcgtt tcatctgtgg tgcaacgggc gctgggtcgg
      601 ttacggccag gacagtcgtt tgccgtctga atttgacctg agcgcatttt tacgcgccgg
      661 agaaaaccgc ctcgcggtga tggtgctgcg ctggagtgac ggcagttatc tggaagatca
      721 ggatatgtgg cggatgagcg gcattttccg tgacgtctcg ttgctgcata aaccgactac
      781 acaaatcagc gatttccatg ttgccactcg ctttaatgat gatttcagcc gcgctgtact
      841 ggaggctgaa gttcagatgt gcggcgagtt gcgtgactac ctacgggtaa cagtttcttt
      901 atggcagggt gaaacgcagg tcgccagcgg caccgcgcct ttcggcggtg aaattatcga
      961 tgagcgtggt ggttatgccg atcgcgtcac actacgtctg aacgtcgaaa acccgaaact
     1021 gtggagcgcc gaaatcccga atctctatcg tgcggtggtt gaactgcaca ccgccgacgg
     1081 cacgctgatt gaagcagaag cctgcgatgt cggtttccgc gaggtgcgga ttgaaaatgg
     1141 tctgctgctg ctgaacggca agccgttgct gattcgaggc gttaaccgtc acgagcatca
     1201 tcctctgcat ggtcaggtca tggatgagca gacgatggtg caggatatcc tgctgatgaa
     1261 gcagaacaac tttaacgccg tgcgctgttc gcattatccg aaccatccgc tgtggtacac
     1321 gctgtgcgac cgctacggcc tgtatgtggt ggatgaagcc aatattgaaa cccacggcat
     1381 ggtgccaatg aatcgtctga ccgatgatcc gcgctggcta ccggcgatga gcgaacgcgt
     1441 aacgcgaatg gtgcagcgcg atcgtaatca cccgagtgtg atcatctggt cgctggggaa
     1501 tgaatcaggc cacggcgcta atcacgacgc gctgtatcgc tggatcaaat ctgtcgatcc
     1561 ttcccgcccg gtgcagtatg aaggcggcgg agccgacacc acggccaccg atattatttg
     1621 cccgatgtac gcgcgcgtgg atgaagacca gcccttcccg gctgtgccga aatggtccat
     1681 caaaaaatgg ctttcgctac ctggagagac gcgcccgctg atcctttgcg aatacgccca
     1741 cgcgatgggt aacagtcttg gcggtttcgc taaatactgg caggcgtttc gtcagtatcc
     1801 ccgtttacag ggcggcttcg tctgggactg ggtggatcag tcgctgatta aatatgatga
     1861 aaacggcaac ccgtggtcgg cttacggcgg tgattttggc gatacgccga acgatcgcca
     1921 gttctgtatg aacggtctgg tctttgccga ccgcacgccg catccagcgc tgacggaagc
     1981 aaaacaccag cagcagtttt tccagttccg tttatccggg caaaccatcg aagtgaccag
     2041 cgaatacctg ttccgtcata gcgataacga gctcctgcac tggatggtgg cgctggatgg
     2101 taagccgctg gcaagcggtg aagtgcctct ggatgtcgct ccacaaggta aacagttgat
     2161 tgaactgcct gaactaccgc agccggagag cgccgggcaa ctctggctca cagtacgcgt
     2221 agtgcaaccg aacgcgaccg catggtcaga agccgggcac atcagcgcct ggcagcagtg
     2281 gcgtctggcg gaaaacctca gtgtgacgct ccccgccgcg tcccacgcca tcccgcatct
     2341 gaccaccagc gaaatggatt tttgcatcga gctgggtaat aagcgttggc aatttaaccg
     2401 ccagtcaggc tctctttcac agatgtggat tggcgataaa aaccaactgc tgacgccgct
     2461 gcgcgatcag ttcacccgtg caccgctgga taacgacatt ggcgtaagtg aagcgacccg
     2521 cattgaccct aacgcctggg tcgaacgctg gaaggcggcg ggccattacc aggccgaagc
     2581 agcgttgttg cagtgcacgg cagatacact tgctgatgcg gtgctgatta cgaccgctca
     2641 cgcgtggcag catcagggga aaaccttatt tatcagccgg aaaacctacc ggattgatgg
     2701 tagtggtcaa atggcgatta ccgttgatgt tgaagtggcg agcgatacac cgcatccggc
     2761 gcggattggc ctgaactgcc agctggcgca ggtagcagag cgggtaaact ggctcggatt
     2821 agggccgcaa gaaaactatc ccgaccgcct tactgccgcc tgttttgacc gctgggatct
     2881 gccattgtca gacatgtata ccccgtacgt cttcccgagc gaaaacggtc tgcgctgcgg
     2941 gacgcgcgaa ttgaattatg gcccacacca gtggcgcggc gacttccagt tcaacatcag
     3001 ccgctacagt caacagcaac tgatggaaac cagccatcgc catctgctgc acgcggaaga
     3061 aggcacatgg ctgaatatcg acggtttcca tatggggatt ggtggcgacg actcctggag
     3121 cccgtcagta tcggcggaat tccagctgag cgccggtcgc taccattacc agttggtctg
     3181 gtgtcaaaaa gcggccgctc gagtctaata ctagagccag gcatcaaata aaacgaaagg
     3241 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     3301 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagtcccttg
     3361 catttacatt ttgaaacatc tatagcgata aatgaaacat cttaaaagtt ttagtatcat
     3421 attcgtgttg gattattctg catttttggg gagaatggac ttactagagt cacacaggac
     3481 ggccggatga gcacaaaaaa gaaaccatta acacaagagc agcttgagga cgcacgtcgc
     3541 cttaaagcaa tttatgaaaa aaagaaaaat gaacttggct tatcccagga atctgtcgca
     3601 gacaagatgg ggatggggca gtcaggcgtt ggtgctttat ttaatggcat caatgcatta
     3661 aatgcttata acgccgcatt gcttgcaaaa attctcaaag ttagcgttga agaatttagc
     3721 ccttcaatcg ccagagaaat ctacgagatg tatgaagcgg ttagtatgca gccgtcactt
     3781 agaagtgagt atgagtaccc tgttttttct catgttcagg cagggatgtt ctcacctgag
     3841 cttagaacct ttaccaaagg tgatgcggag agatgggtaa gcacaaccaa aaaagccagt
     3901 gattctgcat tctggcttga ggttgaaggt aattccatga ccgcaccaac aggctccaag
     3961 ccaagctttc ctgacggaat gttaattctc gttgaccctg agcaggctgt tgagccaggt
     4021 gatttctgca tagccagact tgggggtgat gagtttacct tcaagaaact gatcagggat
     4081 agcggtcagg tgtttttaca accactaaac ccacagtacc caatgatccc atgcaatgag
     4141 agttgttccg ttgtggggaa agttatcgct agtcagtggc ctgaagagac gtttggcgct
     4201 gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta gtgtagatca
     4261 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     4321 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
     4381 ggcctttctg cgtttatata ctagtagcgg ccgctgcagt ccggcaaaaa agggcaaggt
     4441 gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta cttcgcgtta tgcaggcttc
     4501 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     4561 aaaggcggta atctcgaggt tacattgtcg atctgttcat ggtgaacagc tttaaatgca
     4621 ccaaaaactc gtaaaagctc tgatgtatct atctttttta caccgttttc atctgtgcat
     4681 atggacagtt ttccctttga tatctaacgg tgaacagttg ttctactttt gtttgttagt
     4741 cttgatgctt cactgataga tacaagagcc ataagaacct cagatccttc cgtatttagc
     4801 cagtatgttc tctagtgtgg ttcgttgttt ttgcgtgagc catgagaacg aaccattgag
     4861 atcatgctta ctttgcatgt cactcaaaaa ttttgcctca aaactggtga gctgaatttt
     4921 tgcagttaaa gcatcgtgta gtgtttttct tagtccgtta cgtaggtagg aatctgatgt
     4981 aatggttgtt ggtattttgt caccattcat ttttatctgg ttgttctcaa gttcggttac
     5041 gagatccatt tgtctatcta gttcaacttg gaaaatcaac gtatcagtcg ggcggcctcg
     5101 cttatcaacc accaatttca tattgctgta agtgtttaaa tctttactta ttggtttcaa
     5161 aacccattgg ttaagccttt taaactcatg gtagttattt tcaagcatta acatgaactt
     5221 aaattcatca aggctaatct ctatatttgc cttgtgagtt ttcttttgtg ttagttcttt
     5281 taataaccac tcataaatcc tcatagagta tttgttttca aaagacttaa catgttccag
     5341 attatatttt atgaattttt ttaactggaa aagataaggc aatatctctt cactaaaaac
     5401 taattctaat ttttcgcttg agaacttggc atagtttgtc cactggaaaa tctcaaagcc
     5461 tttaaccaaa ggattcctga tttccacagt tctcgtcatc agctctctgg ttgctttagc
     5521 taatacacca taagcatttt ccctactgat gttcatcatc tgagcgtatt ggttataagt
     5581 gaacgatacc gtccgttctt tccttgtagg gttttcaatc gtggggttga gtagtgccac
     5641 acagcataaa attagcttgg tttcatgctc cgttaagtca tagcgactaa tcgctagttc
     5701 atttgctttg aaaacaacta attcagacat acatctcaat tggtctaggt gattttaatc
     5761 actataccaa ttgagatggg ctagtcaatg ataattacat gtccttttcc tttgagttgt
     5821 gggtatctgt aaattctgct agacctttgc tggaaaactt gtaaattctg ctagaccctc
     5881 tgtaaattcc gctagacctt tgtgtgtttt ttttgtttat attcaagtgg ttataattta
     5941 tagaataaag aaagaataaa aaaagataaa aagaatagat cccagccctg tgtataactc
     6001 actactttag tcagttccgc agtattacaa aaggatgtcg caaacgctgt ttgctcctct
     6061 acaaaacaga ccttaaaacc ctaaaggctt aagtagcacc ctcgcaagct cgggcaaatc
     6121 gctgaatatt ccttttgtct ccgaccatca ggcacctgag tcgctgtctt tttcgtgaca
     6181 ttcagttcgc tgcgctcacg gctctggcag tgaatggggg taaatggcac tacaggcgcc
     6241 ttttatggat tcatgcaagg aaactaccca taatacaaga aaagcccgtc acgggcttct
     6301 cagggcgttt tatggcgggt ctgctatgtg gtgctatctg actttttgct gttcagcagt
     6361 tcctgccctc tgattttcca gtctgaccac ttcggattat cccgtgacag gtcattcaga
     6421 ctggctaatg cacccagtaa ggcagcggta tcatcaacag gcttacccgt cttactgtcc
     6481 ctagtgcttg gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa
     6541 tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgta
     6601 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     6661 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     6721 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     6781 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     6841 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     6901 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     6961 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     7021 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     7081 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     7141 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     7201 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     7261 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     7321 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     7381 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     7441 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     7501 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     7561 taaacaaata ggggttccgc gcacatttcc catggtgcca cctgacgtct aagaaaccat
     7621 tattatcatg acattaacct ataaaaatag gcgtatcacg aggcagaatt tcagataaaa
     7681 aaaatcctta gctttcgcta aggatgattt ctggaattcg cggccgcttc tagag
//
