LOCUS       sequence-14386-e        6941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6941)
  AUTHORS   Tabor JJ, Levskaya A, Voigt CA
  TITLE     Multichromatic control of gene expression in Escherichia coli.
  JOURNAL   J Mol Biol. 2011 Jan 14. 405(2):315-24.
  PUBMED    21035461
REFERENCE   2  (bases 1 to 6941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2011 Jan 14. 405(2):315-24."
COMMENT     Sequence Label: pJT118
FEATURES             Location/Qualifiers
     source          1..6941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(2936..5980)
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGSLSQMWIGDKNQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQK"
     primer_bind     5956..5978
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ctaagctcga ggcaaatggt tatagcgttt taaacagata gtaactgttg ttccctggtc
       61 aactgcactt tttagcaaga tactgccact gtgtaagtca acacatttct tggcaaccat
      121 taaaccgagt cctgttcccg taatatttct gacatttttg ccccgatgaa agggttcaaa
      181 aatttgcttt tggtcctctg gcgaaatgcc aatgccctgg tcggtgactt caaaaataat
      241 ctgttccgaa tctaggctta gggcaatttt aatctgccct cccccgggag agtatttaat
      301 cgcattagat aacagattag ataaaataga ccgcactaac ctttcatcca ccaatgcctt
      361 cgtatcttga gcgctacaaa taaagtcaaa ataatattgg tcactgacac ttaattgaat
      421 ttcttcgata aattgctgga acaataattt caaatctaac caattaggat taaattccaa
      481 tttgcccgct tcggcacggt taatgattaa aatatcatcc aggagctgta ccatattttt
      541 cacggaattt tgaatacggt gtaagttccg gctacgctta tcgggatcaa gccaggccac
      601 ttcagaattt tccagtaatt gggcagcagc taaggccgta ctgaggggag tacgaaattc
      661 atgggaagcc atggagaaaa aacgagtttt taggcggctt aattcttttt cccgttctaa
      721 agctaggcgg acttcctccg tgcgtcggcg ctcagtgaca tccctttgca cagcaatcca
      781 atgggtgtaa aaacctgttt tattggcaac gggcaccaga ctaaattcca cccaaaactc
      841 actgccatcc ttacgataat taatcacttc aacggtaact gattgccatt gactaatggc
      901 ttgccgcacc ctatctaatt cagtgcgact agtttttggt ccctgtaaaa ctcggggggt
      961 tttgcctagc atttcttcag cagtataacc ggtgatttta gtaaatgctt cattgacata
     1021 gagaattctc ggcccgggat catcaatggg ctccgcttcc gtaatcacca ccgcatcatt
     1081 agtattaacc acaacggatt gtagcaaacg aatttgttct gcctgccgct tttgctcggt
     1141 aatatcggcg acaaccgcca ccaatccggc aatattttct tcactatcca ataggggagc
     1201 agcggaaagg acaatttcaa tccaactacc atcttttttt tgacatttta attctaggct
     1261 aggggaaacc atgcctgata aaactttctg tttaaattgc tgataatctt ccagcaaaat
     1321 gttggatgtt aataattctg gatgggcaat aatttctgtt tctgtccaac caaaaatacg
     1381 ttctgctgta ggattccaaa tctgaatttg gtctgctaaa ttaagggtaa aaatacccct
     1441 gggggaggcc gcaatcaggg attgcagagt atggttagtg tggcggagag cggcttccgt
     1501 tttttgtcgc tcactgattt ccattcttag ggattgattg gtggcggcaa gttgctgggt
     1561 gcgtttttcg actcggtttt ccaaatcttt attgagttgc tgtaattgct catataattc
     1621 cgattgttgg atggcgatcg cgacctgatt ggctagctgt ttcattaact ccacttccca
     1681 cggttgccat ggccgggtaa aagcacattg atgggtaatt aacagccccc aaagataggg
     1741 aaattctgat tcattatcta gggaagaagc acgattatgt tgaagaatgg gcactactaa
     1801 ttttgatttc acgccaaatt gtttgacgaa atcagctagg caaatctcta tgtcatcctg
     1861 gtcaatgtca ttaatggccc gtactttacc tttggtgtag gcttgatggt attcaacggg
     1921 aaaaacttca tcggaaaagg tccgccctaa aatactagga taattggcat tcaccgattc
     1981 cgtaatggcg ctgcccgtgc catcttgcca aatgcgataa attagaactc gatcaacttg
     2041 caacagggtt ttaacttcag cgacggtggt atttaaaacc gtttccaagt ttaaagattg
     2101 gcggatatgt tgggtaattt ggttaataaa tcgttcccgt tgggtttgtt gcttgatgct
     2161 aatttcaatc ctgcggcgtt cttggttttg tctgtgccat aaataacagg tcatggcgat
     2221 cgccagaaaa acaaattcga ttggaattag aaatttgccc atatattagc gaatcccagt
     2281 agaaaaacct tacttaaaat atttttgttt ggcaataatt ttcatgactt ttaacaataa
     2341 agctaaatca tgatccttgg caaaaacgcc gtcaatttgg ggcaggtaac tgcgagcttg
     2401 taccgtgatc tgactgtcgg cacaatccgt aattagtagt agggccggcg gcttagcaga
     2461 caatttttta ataatggtca ctaaatctaa agtagctgaa atatcaattg attcatattt
     2521 aatcccgcaa ccaactagga tcgaatgata ttctccaacg tcaacattgt taaacaaaat
     2581 atcttggaag ttgatagcag aaatttcaaa acttggacat aaaaatcgct tgactgattg
     2641 ataccactgc cgatccattt ctaggatacc aatttttgcc ttcgttttct tgcaggtcag
     2701 ggcagaggct tttttaccgg ctagtttcaa cattggagtc tggcctcaaa tacaaagtct
     2761 gcaaagtctc gaaatattaa accagagaat aaataattaa acgccaaaat atttctagca
     2821 aaaaccccag ttattaaacc gcctaagtcc cccaggaaag ggggatataa cagtatagat
     2881 tttgtcagcc ttcagcttgg ctttaccgtc aaaaaaatta gactcgagcg gccgcttttt
     2941 gacaccagac caactggtaa tggtagcgac cggcgctcag ctggaattcc gccgatactg
     3001 acgggctcca ggagtcgtcg ccaccaatcc ccatatggaa accgtcgata ttcagccatg
     3061 tgccttcttc cgcgtgcagc agatggcgat ggctggtttc catcagttgc tgttgactgt
     3121 agcggctgat gttgaactgg aagtcgccgc gccactggtg tgggccataa ttcaattcgc
     3181 gcgtcccgca gcgcagaccg ttttcgctcg ggaagacgta cggggtatac atgtctgaca
     3241 atggcagatc ccagcggtca aaacaggcgg cagtaaggcg gtcgggatag ttttcttgtg
     3301 gccctaatcc gagccagttt acccgctctg ctacctgcgc cagctggcag ttcaggccaa
     3361 tccgcgccgg atgcggtgta tcgctcgcca cttcaacatc aacggtaatc gccatttgac
     3421 cactaccatc aatccggtag gttttccggc tgataaataa ggttttcccc tgatgctgcc
     3481 acgcgtgagc ggtcgtaatc agcaccgcat cagcaagtgt atctgccgtg cactgcaaca
     3541 acgctgcttc ggcctggtaa tggcccgccg ccttccagcg ttcgacccag gcgttagggt
     3601 caatgcgggt cgcttcactt acgccaatgt cgttatccag cggtgcacgg gtgaactgat
     3661 cgcgcagcgg cgtcagcagt tggtttttat cgccaatcca catctgtgaa agagagcctg
     3721 actggcggtt aaattgccaa cgcttattac ccagctcgat gcaaaaatcc atttcgctgg
     3781 tggtcagatg cgggatggcg tgggacgcgg cggggagcgt cacactgagg ttttccgcca
     3841 gacgccactg ctgccaggcg ctgatgtgcc cggcttctga ccatgcggtc gcgttcggtt
     3901 gcactacgcg tactgtgagc cagagttgcc cggcgctctc cggctgcggt agttcaggca
     3961 gttcaatcaa ctgtttacct tgtggagcga catccagagg cacttcaccg cttgccagcg
     4021 gcttaccatc cagcgccacc atccagtgca ggagctcgtt atcgctatga cggaacaggt
     4081 attcgctggt cacttcgatg gtttgcccgg ataaacggaa ctggaaaaac tgctgctggt
     4141 gttttgcttc cgtcagcgct ggatgcggcg tgcggtcggc aaagaccaga ccgttcatac
     4201 agaactggcg atcgttcggc gtatcgccaa aatcaccgcc gtaagccgac cacgggttgc
     4261 cgttttcatc atatttaatc agcgactgat ccacccagtc ccagacgaag ccgccctgta
     4321 aacggggata ctgacgaaac gcctgccagt atttagcgaa accgccaaga ctgttaccca
     4381 tcgcgtgggc gtattcgcaa aggatcagcg ggcgcgtctc tccaggtagc gaaagccatt
     4441 ttttgatgga ccatttcggc acagccggga agggctggtc ttcatccacg cgcgcgtaca
     4501 tcgggcaaat aatatcggtg gccgtggtgt cggctccgcc gccttcatac tgcaccgggc
     4561 gggaaggatc gacagatttg atccagcgat acagcgcgtc gtgattagcg ccgtggcctg
     4621 attcattccc cagcgaccag atgatcacac tcgggtgatt acgatcgcgc tgcaccattc
     4681 gcgttacgcg ttcgctcatc gccggtagcc agcgcggatc atcggtcaga cgattcattg
     4741 gcaccatgcc gtgggtttca atattggctt catccaccac atacaggccg tagcggtcgc
     4801 acagcgtgta ccacagcgga tggttcggat aatgcgaaca gcgcacggcg ttaaagttgt
     4861 tctgcttcat cagcaggata tcctgcacca tcgtctgctc atccatgacc tgaccatgca
     4921 gaggatgatg ctcgtgacgg ttaacgcctc gaatcagcaa cggcttgccg ttcagcagca
     4981 gcagaccatt ttcaatccgc acctcgcgga aaccgacatc gcaggcttct gcttcaatca
     5041 gcgtgccgtc ggcggtgtgc agttcaacca ccgcacgata gagattcggg atttcggcgc
     5101 tccacagttt cgggttttcg acgttcagac gtagtgtgac gcgatcggca taaccaccac
     5161 gctcatcgat aatttcaccg ccgaaaggcg cggtgccgct ggcgacctgc gtttcaccct
     5221 gccataaaga aactgttacc cgtaggtagt cacgcaactc gccgcacatc tgaacttcag
     5281 cctccagtac agcgcggctg aaatcatcat taaagcgagt ggcaacatgg aaatcgctga
     5341 tttgtgtagt cggtttatgc agcaacgaga cgtcacggaa aatgccgctc atccgccaca
     5401 tatcctgatc ttccagataa ctgccgtcac tccagcgcag caccatcacc gcgaggcggt
     5461 tttctccggc gcgtaaaaat gcgctcaggt caaattcaga cggcaaacga ctgtcctggc
     5521 cgtaaccgac ccagcgcccg ttgcaccaca gatgaaacgc cgagttaacg ccatcaaaaa
     5581 taattcgcgt ctggccttcc tgtagccagc tttcatcaac attaaatgtg agcgagtaac
     5641 aacccgtcgg attctccgtg ggaacaaacg gcggattgac cgtaatggga taggtcacgt
     5701 tggtgtagat gggcgcatcg taaccgtgca tctgccagtt tgaggggacg acgacagtat
     5761 cggcctcagg aagatcgcac tccagccagc tttccggcac cgcttctggt gccggaaacc
     5821 aggcaaagcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     5881 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     5941 taacgccagg gttttcccag tcacgacgtt gtaaaacgac gggatctatc atagataaag
     6001 ttagtaatta aacttaaaag ttgtttaatg tccagccaat aactggtatg tgttggcatt
     6061 aaaaatgcga tcctaacaaa gtaaaattga agaaaagggg gagaaaatcg taaagaaatc
     6121 ggaaagttag ctgatgcgac taaaactgtt acatttcgca acatgaatta acccagacct
     6181 gttcacccct tcaggtaagc tgaggttgat agagaaaagc acaatgggct atgagaattc
     6241 ttttagtgga ggatgatttg ccgctggcgg aaacccttgc tgaagcattg agtgaccagc
     6301 tttacaccgt tgatattgcc accgacgctt ccctcgcctg ggactatgcc tcccgactgg
     6361 aatatgacct cgttattttg gatgtgatgc tgccggagtt ggacgggatt accctctgtc
     6421 aaaaatggcg atcgcacagt tatttaatgc caattttgat gatgacagcc agggatacga
     6481 tcaatgataa aatcacgggc ttggatgcgg gggcggatga ttatgtggtc aagccagtgg
     6541 atttggggga gttatttgcc agggtgcgag ctttgttgcg tcggggttgt gcaacgtgcc
     6601 aaccagtttt agagtggggg ccaatcaggt tggatccaag cacctatgaa gttagttatg
     6661 acaatgaggt tttgtctttg acccgcaagg aatacagcat tctggaatta ctactccgca
     6721 atggccgtcg ggtgctaagt cggagcatga ttatcgatag tatctggaag ttggagagtc
     6781 ccccagagga agatacggtt aaggtgcatg tgcggagttt gcgacaaaaa ttaaaaagtg
     6841 ccggtttatc agcagatgcc attgaaacgg tccatggcat tgggtatcgt ctggccaatt
     6901 taacggaaaa atctttgtgc caagggaaaa actagtctag a
//
