LOCUS       sequence-14396-e        9563 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9563)
  AUTHORS   Wirsing A, Senkel S, Klein-Hitpass L, Ryffel GU
  TITLE     A systematic analysis of the 3'UTR of HNF4A mRNA reveals an 
            interplay of regulatory elements including miRNA target sites.
  JOURNAL   PLoS One. 2011;6(11):e27438. Epub 2011 Nov 30.
  PUBMED    22140441
REFERENCE   2  (bases 1 to 9563)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(11)"; pages: "e27438. Epub 2011 Nov 30"
COMMENT     Sequence Label: pRLCon1-3180
FEATURES             Location/Qualifiers
     source          1..9563
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     726..750
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1005..1024
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1089..1098
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1095..2030
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    5267..5388
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5278..5297)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5332..5351
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      5574..6029
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5661..5680)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5871..5892
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6146..6503
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6146..6337
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6169..6189)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6354..6489
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6554..7348
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6608..6627)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7218..7237
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7412..7460
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(7501..7520)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7620..7642
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7680..7698)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7766..7870
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7871..7939,7940..8731)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8089..8108)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8902..9490
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9391..9410
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc caccatggct tccaaggtgt acgaccccga gcaacgcaaa cgcatgatca
     1141 ctgggcctca gtggtgggct cgctgcaagc aaatgaacgt gctggactcc ttcatcaact
     1201 actatgattc cgagaagcac gccgagaacg ccgtgatttt tctgcatggt aacgctgcct
     1261 ccagctacct gtggaggcac gtcgtgcctc acatcgagcc cgtggctaga tgcatcatcc
     1321 ctgatctgat cggaatgggt aagtccggca agagcgggaa tggctcatat cgcctcctgg
     1381 atcactacaa gtacctcacc gcttggttcg agctgctgaa ccttccaaag aaaatcatct
     1441 ttgtgggcca cgactggggg gcttgtctgg cctttcacta ctcctacgag caccaagaca
     1501 agatcaaggc catcgtccat gctgagagtg tcgtggacgt gatcgagtcc tgggacgagt
     1561 ggcctgacat cgaggaggat atcgccctga tcaagagcga agagggcgag aaaatggtgc
     1621 ttgagaataa cttcttcgtc gagaccatgc tcccaagcaa gatcatgcgg aaactggagc
     1681 ctgaggagtt cgctgcctac ctggagccat tcaaggagaa gggcgaggtt agacggccta
     1741 ccctctcctg gcctcgcgag atccctctcg ttaagggagg caagcccgac gtcgtccaga
     1801 ttgtccgcaa ctacaacgcc taccttcggg ccagcgacga tctgcctaag atgttcatcg
     1861 agtccgaccc tgggttcttt tccaacgcta ttgtcgaggg agctaagaag ttccctaaca
     1921 ccgagttcgt gaaggtgaag ggcctccact tcagccagga ggacgctcca gatgaaatgg
     1981 gtaagtacat caagagcttc gtggagcgcg tgctgaagaa cgagcagtaa ttctagttag
     2041 caagccgctg gggcttgggg gctccactgg ctccccccag ccccctaaga gagcacctgg
     2101 tgatcacgtg gtcacggcaa aggaagacgt gatgccagga ccagtcccag agcaggaatg
     2161 ggaaggatga agggcccgag aacatggcct aagggccaca tcccactgcc acccttgacg
     2221 ccctgctctg gataacaaga ctttgacttg gggagacctc tactgccttg gacaactttt
     2281 ctcatgttga agccactgcc ttcaccttca ccttcatcca tgtccaaccc ccgacttcat
     2341 cccaaaggac agccgcctgg agatgacttg aggccttact taaacccagc tcccttcttc
     2401 cctagcctgg tgcttctcct ctcctagccc ctgtcatggt gtccagacag agccctgtga
     2461 ggctgggtcc aattgtggca cttggggcac cttgctcctc cttctgctgc tgcccccacc
     2521 tctgctgcct ccctctgctg tcaccttgct cagccatccc gtcttctcca acaccacctc
     2581 tccagaggcc aaggaggcct tggaaacgat tcccccagtc attctgggaa catgttgtaa
     2641 gcactgactg ggaccaggca ccaggcaggg tctagaaggc tgtggtgagg gaagacgcct
     2701 ttctcctcca acccaacctc atcctccttc ttcagggact tgggtgggta cttgggtgag
     2761 gatccctgaa ggccttcaac ccgagaaaac aaacccaggt tggcgactgc aacaggaact
     2821 tggagtggag aggaaaagca tcagaaagag gcagaccatc caccaggcct ttgagaaagg
     2881 gtagaattct ggctggtaga gcaggtgaga tgggacattc caaagaacag cctgagccaa
     2941 ggcctagtgg tagtaagaat ctagcaagaa ttgaggaaga atggtgtggg agagggatga
     3001 tgaagagaga gagggcctgc tggagagcat agggtctgga acaccaggct gaggtcctga
     3061 tcagcttcaa ggagtatgca gggagctggg cttccagaaa atgaacacag cagttctgca
     3121 gaggacggga ggctggaagc tgggaggtca ggtggggtgg atgatataat gcgggtgaga
     3181 gtaatgaggc ttggggctgg agaggacaag atgggtaaac cctcacatca gagtgacatc
     3241 caggaggaat aagctcccag ggcctgtctc aagctcttcc ttactcccag gcactgtctt
     3301 aaggcatctg acatgcatca tctcatttaa tcctcccttc ctccctatta acctagagat
     3361 tgtttttgtt ttttattctc ctcctccctc cccgccctca cccgccccac tccctcctaa
     3421 cctagagatt gttacagaag ctgaaattgc gttctaagag gtgaagtgat tttttttctg
     3481 aaactcacac aactaggaag tggctgagtc aggacttgaa cccaggtctc cctggatcag
     3541 aacaggagct cttaactaca gtggctgaat agcttctcca aaggctccct gtgttctcac
     3601 cgtgatcaag ttgaggggct tccggctccc ttctacagcc tcagaaacca gactcgttct
     3661 tctgggaacc ctgcccactc ccaggaccaa gattggcctg aggctgcact aaaattcact
     3721 tagggtcgag catcctgttt gctgataaat attaaggaga attcatgact cttgacagct
     3781 tttctctctt cactccccaa gtcaagggga ggggtggcag gggtctgttt cctggaagtc
     3841 aggctcatct ggcctgttgg catgggggtg ggacagtgtg cacagtgtgg gggcagggga
     3901 gggctaagca ggcctgggtt tgagggctgc tccggagacc gtcactccag gtgcattctg
     3961 gaagcattag accccaggat ggagcgacca gcatgtcatc catgtggaat cttggtggct
     4021 ttgaggacat tctggaaaat gccactgacc agtgtgaaca aaagggatgt gttatggggc
     4081 tggaggtgtg attaggtagg agggaaactg ttggaccgac tcctgccccc tgctcaacac
     4141 tgacccctct gagtggttgg aggcagtgcc ccagtgccca gaaatcccac cattagtgat
     4201 tgttttttat gagaaagagg cgtggagaag tattggggca atgtgtcagg gaggaatcac
     4261 cacatcccta cggcagtccc agccaagccc ccaatcccag cggagactgt gccctgctca
     4321 gagctcccaa gccttccccc accacctcac tcaagtgccc ctgaaatccc tgccagacgg
     4381 ctcagcctgg tctgcggtaa ggcagggagg ctggaaccat ttctgggcat tgtggtcatt
     4441 cccactgtgt tcctccacct cctccctcca gcgttgctca gacctctgtc ttgggagaaa
     4501 ggttgagata agaatgtccc atggagtgcc gtgggcaaca gtggcccttc atgggaacaa
     4561 tctgttggag cagggggtca gttctctgct gggaatctac ccctttctgg aggagaaacc
     4621 cattccacct taataacttt attgtaatgt gagaaacaca aaacaaagtt tacttttttg
     4681 actctaagct gacatgatat tagaaaatct ctcgctctct tttttttttt tttttttttt
     4741 tttggctact tgagttgtgg tcctaaaaca taaaatctga tggacaaaca gagggttgct
     4801 ggggggacaa gcgtgggcac aatttcccca ccaagacacc ctgatcttca ggcgggtctc
     4861 aggagcttct aaaaatccgc atggctctcc tgagagtgga cagaggagag gagagggtca
     4921 gaaatgaacg ctcttctatt tcttgtcatt accaagccaa ttacttttgc caaatttttc
     4981 tgtgatctgc cctgattaag atgaattgtg aaatttacat caagcaatta tcaaagcggg
     5041 ctgggtccca tcagaacgac ccacatcttt ctgtgggtgt gaatgtcatt aggtcttgcg
     5101 ctgacccctg agcccccatc actgccgcct gatggggcaa agaaacaaaa aacatttctt
     5161 actcttctgt gttttaacaa aagtttataa aacaaaataa atggcgcata tgttttctaa
     5221 gcggccgctt ccctttagtg agggttaatg cttcgagcag acatgataag atacattgat
     5281 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     5341 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     5401 tgcattcatt ttatgtttca ggttcagggg gagatgtggg aggtttttta aagcaagtaa
     5461 aacctctaca aatgtggtaa aatccgataa ggatcgatcc gggctggcgt aatagcgaag
     5521 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggacgcgcc
     5581 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     5641 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     5701 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     5761 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     5821 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     5881 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     5941 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     6001 ttttaacaaa atattaacgc ttacaatttc ctgatgcggt attttctcct tacgcatctg
     6061 tgcggtattt cacaccgcat acgcggatct gcgcagcacc atggcctgaa ataacctctg
     6121 aaagaggaac ttggttaggt accttctgag gcggaaagaa ccagctgtgg aatgtgtgtc
     6181 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     6241 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     6301 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     6361 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     6421 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     6481 ttggaggcct aggcttttgc aaaaagcttg attcttctga cacaacagtc tcgaacttaa
     6541 ggctagagcc accatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     6601 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     6661 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     6721 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     6781 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     6841 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     6901 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     6961 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     7021 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     7081 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     7141 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     7201 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     7261 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     7321 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     7381 gcgacgccca acctgccatc acgatggccg caataaaata tctttatttt cattacatct
     7441 gtgtgttggt tttttgtgtg aatcgatagc gataaggatc cgcgtatggt gcactctcag
     7501 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     7561 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     7621 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     7681 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     7741 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     7801 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     7861 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     7921 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     7981 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     8041 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     8101 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     8161 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     8221 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     8281 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     8341 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     8401 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     8461 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     8521 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     8581 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     8641 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     8701 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     8761 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     8821 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     8881 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     8941 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     9001 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     9061 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     9121 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     9181 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     9241 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     9301 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     9361 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     9421 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     9481 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     9541 tgctcacatg gctcgacaga tct
//
