LOCUS       sequence-14421-e        1128 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Hibbs RE, Gouaux E
  TITLE     Principles of activation and permeation in an anion-selective 
            Cys-loop receptor.
  JOURNAL   Nature. 2011 Jun 2. 474(7349):54-60.
  PUBMED    21572436
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2011 Jun 2. 474(7349):54-60."
COMMENT     Sequence Label: pGEM-GluCl_cryst
FEATURES             Location/Qualifiers
     source          1..1128
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1090..1113
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gcggccgcca ccatggccac ctggattgtg ggcaagttga taattgccag cttgatactc
       61 ggaatccaag cgtccgactc aaagatcctt gcgcacctct ttacgtcggg ttacgatttc
      121 cgtgttcgtc caccgactga caacggagga cccgtcgtcg tgtccgtgaa tatgctgctg
      181 cgtaccatat cgaaaatcga cgtagtgaac atggagtact cagcacagct cacgttgaga
      241 gaatcatgga ttgataaacg tctcagctat ggcgtgaaag gcgacggcca acctgacttc
      301 gtgatcctta ctgtgggtca ccaaatctgg atgcccgaca cgtttttccc aaatgagaaa
      361 caagcctaca agcataccat agacaaacct aacgtgttga tcaggatcca caacgacgga
      421 accgtgctgt actccgtgcg catcagcctg gttctgagtt gtcccatgta tttgcaatac
      481 taccccatgg acgttcagca gtgcagtatt gacctggcat catatgctta taccacaaag
      541 gacatcgaat acctgtggaa ggagcattca cctctccagc tgaaggtcgg tctgtcaagc
      601 tctctgcctt cctttcagtt gacaaacaca tctaccacct attgcactag cgtcacaaac
      661 accggtatct actcatgcct gcgcaccacg atccagctca aaagagaatt cagtttctac
      721 ctcttgcagc tttacatccc ttcttgcatg ttggtaatcg tctcttgggt tagcttctgg
      781 ttcgatcgta ctgcaattcc cgcacgcgta acgctgggcg tgacaactct gctgaccatg
      841 accgctcaga gtgccggaat caattctcaa ctcccacccg tatcctatat caaggctatt
      901 gatgtctgga tcggagcttg tatgacattc attttctgtg ctctcttgga attcgctttg
      961 gtaaaccaca ttgctaacgc tggtactact gaatggaacg atatctctaa gagggtagat
     1021 ctcatctcgc gcgccttgtt ccctgtcttg ttcttcgtgt tcaatattct ttactggagc
     1081 aggttcggac atcaccacca tcaccatcac cattaaggta ccaagctt
//
