LOCUS       sequence-14423-e        8004 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8004)
  AUTHORS   Wycuff DR, Matthews KS
  TITLE     Generation of an AraC-araBAD promoter-regulated T7 expression 
            system.
  JOURNAL   Anal Biochem. 2000 Jan 1. 277(1):67-73.
  PUBMED    10610690
REFERENCE   2  (bases 1 to 8004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem. 2000 Jan 1. 277(1):67-73."
COMMENT     Sequence Label: pTARA
FEATURES             Location/Qualifiers
     source          1..8004
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      337..791
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(424..443)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(1346..2005)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1920..1939
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2006..2108)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(2500..2517)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2634..3179)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(2714..2733)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3251..3270
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(3450..4328)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        4355..4639
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     4562..4581
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     primer_bind     complement(7399..7416)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      7569..7655
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      7747..7774
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        7793..7884
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
       61 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
      121 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
      181 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatga
      241 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
      301 agaccaagtt tactcatata tactttagat tgatttacgc gccctgtagc ggcgcattaa
      361 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
      421 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
      481 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
      541 aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
      601 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa acttgaacaa
      661 cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg atttcggcca
      721 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
      781 gtttacaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
      841 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
      901 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
      961 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     1021 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     1081 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtcaggc
     1141 atttgagaag cacacggtca cactgcttcc ggtagtcaat aaaccggtaa accagcaata
     1201 gacataagcg gctatttaac gaccctgccc tgaaccgacg accgggtcga atttgctttc
     1261 gaatttctgc cattcatccg cttattatca cttattcagg cgtagcacca ggcgtttaag
     1321 ggcaccaata actgccttaa aaaaattacg ccccgccctg ccactcatcg cagtactgtt
     1381 gtaattcatt aagcattctg ccgacatgga agccatcaca gacggcatga tgaacctgaa
     1441 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg
     1501 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
     1561 gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
     1621 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
     1681 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
     1741 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacggaat tccggatgag
     1801 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct
     1861 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag
     1921 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg
     1981 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact
     2041 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt
     2101 gccgatcaac gtctcatttt cgccaaaagt tggcccaggg cttcccggta tcaacaggga
     2161 caccaggatt tatttattct gcgaagtgat cttccgtcac aggtatttat tcggcgcaaa
     2221 gtgcgtcggg tgatgctgcc aacttactga tttagtgtat gatggtgttt ttgaggtgct
     2281 ccagtggctt ctgtttctat cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt
     2341 aacggcaaaa gcaccgccgg acatcagcgc tagcggagtg tatactggct tactatgttg
     2401 gcactgatga gggtgtcagt gaagtgcttc atgtggcagg agaaaaaagg ctgcaccggt
     2461 gcgtcagcag aatatgtgat acaggatata ttccgcttcc tcgctcactg actcgctacg
     2521 ctcggtcgtt cgactgcggc gagcggaaat ggcttacgaa cggggcggag atttcctgga
     2581 agatgccagg aagatactta acagggaagt gagagggccg cggcaaagcc gtttttccat
     2641 aggctccgcc cccctgacaa gcatcacgaa atctgacgct caaatcagtg gtggcgaaac
     2701 ccgacaggac tataaagata ccaggcgttt ccccctggcg gctccctcgt gcgctctcct
     2761 gttcctgcct ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt gtctcattcc
     2821 acgcctgaca ctcagttccg ggtaggcagt tcgctccaag ctggactgta tgcacgaacc
     2881 ccccgttcag tccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccgga
     2941 aagacatgca aaagcaccac tggcagcagc cactggtaat tgatttagag gagttagtct
     3001 tgaagtcatg cgccggttaa ggctaaactg aaaggacaag ttttggtgac tgcgctcctc
     3061 caagccagtt acctcggttc aaagagttgg tagctcagag aaccttcgaa aaaccgccct
     3121 gcaaggcggt tttttcgttt tcagagcaag agattacgcg cagaccaaaa cgatctcaag
     3181 aagatcatct tattaatcag ataaaatatt tgctcatgag cccgaagtgg cgagcccgat
     3241 cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga
     3301 tgccggccac gatgcgtccg gcgtagagga tctgctcatg tttgacagct tatcatcgat
     3361 gcataatgtg cctgtcaaat ggacgaagca gggattctgc aaaccctatg ctactccgtc
     3421 aagccgtcaa ttgtctgatt cgttaccaat tatgacaact tgacggctac atcattcact
     3481 ttttcttcac aaccggcacg gaactcgctc gggctggccc cggtgcattt tttaaatacc
     3541 cgcgagaaat agagttgatc gtcaaaacca acattgcgac cgacggtggc gataggcatc
     3601 cgggtggtgc tcaaaagcag cttcgcctgg ctgatacgtt ggtcctcgcg ccagcttaag
     3661 acgctaatcc ctaactgctg gcggaaaaga tgtgacagac gcgacggcga caagcaaaca
     3721 tgctgtgcga cgctggcgat atcaaaattg ctgtctgcca ggtgatcgct gatgtactga
     3781 caagcctcgc gtacccgatt atccatcggt ggatggagcg actcgttaat cgcttccatg
     3841 cgccgcagta acaattgctc aagcagattt atcgccagca gctccgaata gcgcccttcc
     3901 ccttgcccgg cgttaatgat ttgcccaaac aggtcgctga aatgcggctg gtgcgcttca
     3961 tccgggcgaa agaaccccgt attggcaaat attgacggcc agttaagcca ttcatgccag
     4021 taggcgcgcg gacgaaagta aacccactgg tgataccatt cgcgagcctc cggatgacga
     4081 ccgtagtgat gaatctctcc tggcgggaac agcaaaatat cacccggtcg gcaaacaaat
     4141 tctcgtccct gatttttcac caccccctga ccgcgaatgg tgagattgag aatataacct
     4201 ttcattccca gcggtcggtc gataaaaaaa tcgagataac cgttggcctc aatcggcgtt
     4261 aaacccgcca ccagatgggc attaaacgag tatcccggca gcaggggatc attttgcgct
     4321 tcagccatac ttttcatact cccgccattc agagaagaaa ccaattgtcc atattgcatc
     4381 agacattgcc gtcactgcgt cttttactgg ctcttctcgc taaccaaacc ggtaaccccg
     4441 cttattaaaa gcattctgta acaaagcggg accaaagcca tgacaaaaac gcgtaacaaa
     4501 agtgtctata atcacggcag aaaagtccac attgattatt tgcacggcgt cacactttgc
     4561 tatgccatag catttttatc cataagatta gcggatccta cctgacgctt tttatcgcaa
     4621 ctctctactg tttctccata cccgtttttt tgggctagcg aattcgagct caagaggata
     4681 ccatatgaac acgattaaca tcgctaagaa cgacttctct gacatcgaac tggctgctat
     4741 cccgttcaac actctggctg accattacgg tgagcgttta gctcgcgaac agttggccct
     4801 tgagcatgag tcttacgaga tgggtgaagc acgcttccgc aagatgtttg agcgtcaact
     4861 taaagctggt gaggttgcgg ataacgctgc cgccaagcct ctcatcacta ccctactccc
     4921 taagatgatt gcacgcatca acgactggtt tgaggaagtg aaagctaagc gcggcaagcg
     4981 cccgacagcc ttccagttcc tgcaagaaat caagccggaa gccgtagcgt acatcaccat
     5041 taagaccact ctggcttgcc taaccagtgc tgacaataca accgttcagg ctgtagcaag
     5101 cgcaatcggt cgggccattg aggacgaggc tcgcttcggt cgtatccgtg accttgaagc
     5161 taagcacttc aagaaaaacg ttgaggaaca actcaacaag cgcgtagggc acgtctacaa
     5221 gaaagcattt atgcaagttg tcgaggctga catgctctct aagggtctac tcggtggcga
     5281 ggcgtggtct tcgtggcata aggaagactc tattcatgta ggagtacgct gcatcgagat
     5341 gctcattgag tcaaccggaa tggttagctt acaccgccaa aatgctggcg tagtaggtca
     5401 agactctgag actatcgaac tcgcacctga atacgctgag gctatcgcaa cccgtgcagg
     5461 tgcgctggct ggcatctctc cgatgttcca accttgcgta gttcctccta agccgtggac
     5521 tggcattact ggtggtggct attgggctaa cggtcgtcgt cctctggcgc tggtgcgtac
     5581 tcacagtaag aaagcactga tgcgctacga agacgtttac atgcctgagg tgtacaaagc
     5641 gattaacatt gcgcaaaaca ccgcatggaa aatcaacaag aaagtcctag cggtcgccaa
     5701 cgtaatcacc aagtggaagc attgtccggt cgaggacatc cctgcgattg agcgtgaaga
     5761 actcccgatg aaaccggaag acatcgacat gaatcctgag gctctcaccg cgtggaaacg
     5821 tgctgccgct gctgtgtacc gcaaggacaa ggctcgcaag tctcgccgta tcagccttga
     5881 gttcatgctt gagcaagcca ataagtttgc taaccataag gccatctggt tcccttacaa
     5941 catggactgg cgcggtcgtg tttacgctgt gtcaatgttc aacccgcaag gtaacgatat
     6001 gaccaaagga ctgcttacgc tggcgaaagg taaaccaatc ggtaaggaag gttactactg
     6061 gctgaaaatc cacggtgcaa actgtgcggg tgtcgataag gttccgttcc ctgagcgcat
     6121 caagttcatt gaggaaaacc acgagaacat catggcttgc gctaagtctc cactggagaa
     6181 cacttggtgg gctgagcaag attctccgtt ctgcttcctt gcgttctgct ttgagtacgc
     6241 tggggtacag caccacggcc tgagctataa ctgctccctt ccgctggcgt ttgacgggtc
     6301 ttgctctggc atccagcact tctccgcgat gctccgagat gaggtaggtg gtcgcgcggt
     6361 taacttgctt cctagtgaaa ccgttcagga catctacggg attgttgcta agaaagtcaa
     6421 cgagattcta caagcagacg caatcaatgg gaccgataac gaagtagtta ccgtgaccga
     6481 tgagaacact ggtgaaatct ctgagaaagt caagctgggc actaaggcac tggctggtca
     6541 atggctggct tacggtgtta ctcgcagtgt gactaagcgt tcagtcatga cgctggctta
     6601 cgggtccaaa gagttcggct tccgtcaaca agtgctggaa gataccattc agccagctat
     6661 tgattccggc aagggtctga tgttcactca gccgaatcag gctgctggat acatggctaa
     6721 gctgatttgg gaatctgtga gcgtgacggt ggtagctgcg gttgaagcaa tgaactggct
     6781 taagtctgct gctaagctgc tggctgctga ggtcaaagat aagaagactg gagagattct
     6841 tcgcaagcgt tgcgctgtgc attgggtaac tcctgatggt ttccctgtgt ggcaggaata
     6901 caagaagcct attcagacgc gcttgaacct gatgttcctc ggtcagttcc gcttacagcc
     6961 taccattaac accaacaaag atagcgagat tgatgcacac aaacaggagt ctggtatcgc
     7021 tcctaacttt gtacacagcc aagacggtag ccaccttcgt aagactgtag tgtgggcaca
     7081 cgagaagtac ggaatcgaat cttttgcact gattcacgac tccttcggta ccattccggc
     7141 tgacgctgcg aacctgttca aagcagtgcg cgaaactatg gttgacacat atgagtcttg
     7201 tgatgtactg gctgatttct acgaccagtt cgctgaccag ttgcacgagt ctcaattgga
     7261 caaaatgcca gcacttccgg ctaaaggtaa cttgaacctc cgtgacatct tagagtcgga
     7321 cttcgcgttc gcgtaatcta gagtcgacct gcaggcatgc aagcttggct gttttggcgg
     7381 atgagagaag attttcagcc tgatacagat taaatcagaa cgcagaagcg gtctgataaa
     7441 acagaatttg cctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga
     7501 agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg
     7561 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     7621 gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg
     7681 cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa
     7741 ttaagcagaa ggccatcctg acggatggcc tttttgcgtt tctacaaact cttttgttta
     7801 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     7861 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     7921 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     7981 gatgctgaag atcagttggg tgca
//
