LOCUS       sequence-14445-e        2029 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Kajimoto T, Sawamura S, Tohyama Y, Mori Y, Newton AC
  TITLE     Protein kinase C {delta}-specific activity reporter reveals 
            agonist-evoked nuclear activity controlled by Src family of kinases.
  JOURNAL   J Biol Chem. 2010 Dec 31. 285(53):41896-910.
  PUBMED    20959447
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2010 Dec 31. 285(53):41896-910."
COMMENT     Sequence Label: pcDNA3-deltaCKAR
FEATURES             Location/Qualifiers
     source          1..2029
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(14..16,17..19,20..730)
                     /codon_start=1
                     /product="monomeric super enhanced variant of CFP (Matsuda 
                     et al., 2008)"
                     /label=mseCFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             join(14..16,17..19,20..730)
                     /codon_start=1
                     /product="cyan variant of GFP"
                     /label=CFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             join(14..16,17..19,20..730)
                     /codon_start=1
                     /product="enhanced CFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             join(1307..1309,1310..2023)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 aagcttcgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
       61 ggtcgagctg gacggcgacg taaacggcca caggttcagc gtgtccggcg agggcgaggg
      121 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
      181 gccctggccc accctcgtga ccaccctgac ctggggcgtg cagtgcttca gccgctaccc
      241 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
      301 gcgtaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
      361 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
      421 catcctgggg cacaagctgg agtacaacta catcagccac aacgtctata tcaccgccga
      481 caagcagaag aacggcatca aggcccactt caagatccgc cacaacatcg aggacggcag
      541 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
      601 gcccgacaac cactacctga gcacccagtc caagctgagc aaagacccca acgagaagcg
      661 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactcacg gcatggacga
      721 gctgtacaag gctggtacca agggtaatgg taggttttta actttaaaac cattgcctga
      781 cagcattatt caagaaagcc tggagattca gcaaggtgtg aatccatttt tcattggtag
      841 atccgaggat tgcaattgta aaattgaaga cagtaggttg tctcgagttc attgcttcat
      901 tttcaaaaag aggcatgctg taggcaaaag catgtatgaa tctccggcac aaggtttaga
      961 tgatatttgg tattgccaca ccggaactaa cgtgagctat ttaaataata accgcatgat
     1021 acagggtacg aaattccttt tacaagacgg agatgaaatc aagatcattt gggataaaaa
     1081 caataaattt gtcattggct ttaaagtgga aattaacgat actacaggtc tgtttaacga
     1141 gggattaggt atgttacaag aacaaagagt agtacttaag caaacagccg aagaaaaaga
     1201 tttggtgaaa aagttaggag gatccggagg tcggggtgtg tccagccgta gcactttcgc
     1261 tattgggcag ctccgacagg tgcggggtgg atctggtgga attctcgtga gcaagggcga
     1321 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     1381 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     1441 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcgg
     1501 ctacggcctg atgtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa
     1561 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     1621 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     1681 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     1741 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     1801 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     1861 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc
     1921 caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     1981 cgccgccggg atcactcacg gcatggacga gctgtacaag taatctaga
//
