LOCUS       sequence-14493-e        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Taniguchi N, Carames B, Kawakami Y, Amendt BA, Komiya S, Lotz M
  TITLE     Chromatin protein HMGB2 regulates articular cartilage surface 
            maintenance via beta-catenin pathway.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Sep 29. 106(39):16817-22.
  PUBMED    19805379
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2009 Sep 29. 106(39):16817-22."
COMMENT     Sequence Label: pcDNA3.1 Flag hHMGB1
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             199..222
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(919..937)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(920..937)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(957..974)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 aggctcaatt cgggcggcgg ctgctgctgc ctgtggcccg ggcggctggg agaagccgag
       61 tgttggtgag tgacgcggcg gaggtgtagt ttgacgcggt gtgttacgtg ggggagagaa
      121 taaaactcca gcgagatccg ggccgcgaac gaaagcagtg acggaggagc ttgtaccacc
      181 ggtgactaga tgaccatgga ctacaaggac gacgatgaca agctgcagga attcatgggc
      241 aaaggagatc ctaagaagcc gagaggcaaa atgtcatcat atgcattttt tgtgcaaact
      301 tgtcgggagg agcataagaa gaagcaccca gatgcttcag tcaacttctc agagttttct
      361 aagaagtgct cagagaggtg gaagaccatg tctgctaaag agaaaggaaa atttgaagat
      421 atggcaaagg cggacaaggc ccgttatgaa agagaaatga aaacctatat ccctcccaaa
      481 ggggagacaa aaaagaagtt caaggatccc aatgcaccca agaggcctcc ttcggccttc
      541 ttcctcttct gctctgagta tcgcccaaaa atcaaaggag aacatcctgg cctgtccatt
      601 ggtgatgttg cgaagaaact gggagagatg tggaataaca ctgctgcaga tgacaagcag
      661 ccttatgaaa agaaggctgc gaagctgaag gaaaaatacg aaaaggatat tgctgcatat
      721 cgagctaaag gaaagcctga tgcagcaaaa aagggagttg tcaaggctga aaaaagcaag
      781 aaaaagaagg aagaggagga agatgaggaa gatgaagagg atgaggagga ggaggaagat
      841 gaagaagatg aagatgaaga agaagatgat gatgatgaat aagtcgacgc gtctgcagaa
      901 gcttctagag ggccctattc tatagtgtca cctaaatgct agagctcgct gatcagcctc
      961 gactgtgcct tctagttgcc agccatctgt tgttgcccct cccccggctg cctaccgcgg
//
