LOCUS       sequence-14494-e         985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Taniguchi N, Carames B, Kawakami Y, Amendt BA, Komiya S, Lotz M
  TITLE     Chromatin protein HMGB2 regulates articular cartilage surface 
            maintenance via beta-catenin pathway.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Sep 29. 106(39):16817-22.
  PUBMED    19805379
REFERENCE   2  (bases 1 to 985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2009 Sep 29. 106(39):16817-22."
COMMENT     Sequence Label: pcDNA3.1 Flag mHMGB2
FEATURES             Location/Qualifiers
     source          1..985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             201..224
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(885..903)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(886..903)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(923..940)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 aacttcattt tcgggcgggc ggctgctgct gcctgtggcc cgggcggctg ggagaagccg
       61 agtgttggtg agtgacgcgg cggaggtgta gtttgacgcg gtgtgttacg tgggggagag
      121 aataaaactc cagcgagatc cgggccgcga acgaaagcag tgacggagga gcttgtacca
      181 ccggtgacta gatgaccatg gactacaagg acgacgatga caagctgcag gaattcatgg
      241 gcaagggtga ccccaacaag ccgcggggca aaatgtcctc gtacgccttc ttcgtgcaga
      301 cctgccgcga ggagcacaag aagaagcatc ccgactcgtc ggtgaacttc gccgagttct
      361 ccaagaaatg ctccgagaga tggaagacca tgtctgcaaa ggaaaagtcc aagtttgaag
      421 atttggccaa gagcgacaaa gctcgttatg acagggagat gaagaactat gttcctccca
      481 aaggggataa gaaaggaaag aaaaaagacc ccaatgctcc gaagagacca ccgtctgcct
      541 tcttcctgtt ttgctctgaa aatcgcccaa agatcaaaat tgaacaccca ggcctgtcta
      601 ttggagatac tgcgaagaaa ctgggtgaga tgtggtctga gcaatctgcc aaagataaac
      661 aaccgtatga gcagaaagca gctaaactaa aggagaagta tgaaaaggat attgctgcat
      721 accgtgccaa gggcaaaagt gaagcaggaa agaagggtcc tggtaggcca acaggctcaa
      781 agaagaagaa cgaaccagaa gatgaggagg aggaagaaga ggaggaagag gaggaagatg
      841 aagaggaaga agaggaggat gaagaataat ctagagggcc ctattctata gtgtcaccta
      901 aatgctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
      961 gcccctcccc cggctacgag gtggg
//
