LOCUS       sequence-14495-e       11859 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11859)
  AUTHORS   Citri A, Bhattacharyya S, Ma C, Morishita W, Fang S, Rizo J, Malenka
            RC
  TITLE     Calcium binding to PICK1 is essential for the intracellular 
            retention of AMPA receptors underlying long-term depression.
  JOURNAL   J Neurosci. 2010 Dec 8. 30(49):16437-52.
  PUBMED    21147983
REFERENCE   2  (bases 1 to 11859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2010 Dec 8. 30(49):16437-52."
COMMENT     Sequence Label: GFP-PICK
FEATURES             Location/Qualifiers
     source          1..11859
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     2790..2809
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(3009..3028)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3010..3028)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3035..3052)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3035..3051)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3044..3066)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        3176..4386
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4223..4242
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      4484..4493
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4490..4492,4493..4495,4496..5206)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4490..4492,4493..4495,4496..5206)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4535..4556)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4796..4815)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5143..5164
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5207..5233
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    6551..7139
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6604..6624)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7022..7033)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7142..7158)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7143..7159)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7664..7844
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7870..7887)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7876..8100
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8146..8574
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8233..8252)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8443..8464
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8583..8603)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8588..8917
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8768..8903
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8830..8849
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8965..9012
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9031..9405
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9535..9656
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9572..9591)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9626..9645
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9705..9721)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9705..9721)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9718..9740)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9729..9745
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9753..9759,9760..9777,9778..9783))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9798..9819
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9936..9953)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10107..10695)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10187..10206)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10866..11657,11658..11726))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11489..11508
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11727..11831)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatcg gaattcgaac gctgacgtcg acggatcggg agatcccccg
     2641 ggctgcagga attcgaacgc tgacgtcatc aacccgctcc aaggaatcgc gggcccagtg
     2701 tcactaggcg ggaacaccca gcgcgcgtgc gccctggcag gaagatggct gtgagggaca
     2761 ggggagtggc gccctgcaat atttgcatgt cgctatgtgt tctgggaaat caccataaac
     2821 gtgaaatgtc tttggatttg ggaatcttat aagttcccta tcagtgatag ggatccctat
     2881 gagtaccgcc ttatcctttc aagagaagga taaggcggta ctcatagttt tctagcccca
     2941 agggcgaatt ctgcagatat ccatcacact ggcggccgct cgagcatgca tctagagggc
     3001 ccaattcgcc ctatagtgag tcgtattaca attcactggc cgtcgtttta caacgtcgtg
     3061 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     3121 gctggcgtaa tagcgaagag gcccgcaccg attaacccgt gtcggctcca gatctggcct
     3181 ccgcgccggg ttttggcgcc tcccgcgggc gcccccctcc tcacggcgag cgctgccacg
     3241 tcagacgaag ggcgcaggag cgttcctgat ccttccgccc ggacgctcag gacagcggcc
     3301 cgctgctcat aagactcggc cttagaaccc cagtatcagc agaaggacat tttaggacgg
     3361 gacttgggtg actctagggc actggttttc tttccagaga gcggaacagg cgaggaaaag
     3421 tagtcccttc tcggcgattc tgcggaggga tctccgtggg gcggtgaacg ccgatgatta
     3481 tataaggacg cgccgggtgt ggcacagcta gttccgtcgc agccgggatt tgggtcgcgg
     3541 ttcttgtttg tggatcgctg tgatcgtcac ttggtgagtt gcgggctgct gggctggccg
     3601 gggctttcgt ggccgccggg ccgctcggtg ggacggaagc gtgtggagag accgccaagg
     3661 gctgtagtct gggtccgcga gcaaggttgc cctgaactgg gggttggggg gagcgcacaa
     3721 aatggcggct gttcccgagt cttgaatgga agacgcttgt aaggcgggct gtgaggtcgt
     3781 tgaaacaagg tggggggcat ggtgggcggc aagaacccaa ggtcttgagg ccttcgctaa
     3841 tgcgggaaag ctcttattcg ggtgagatgg gctggggcac catctgggga ccctgacgtg
     3901 aagtttgtca ctgactggag aactcgggtt tgtcgtctgg ttgcgggggc ggcagttatg
     3961 cggtgccgtt gggcagtgca cccgtacctt tgggagcgcg cgcctcgtcg tgtcgtgacg
     4021 tcacccgttc tgttggctta taatgcaggg tggggccacc tgccggtagg tgtgcggtag
     4081 gcttttctcc gtcgcaggac gcagggttcg ggcctagggt aggctctcct gaatcgacag
     4141 gcgccggacc tctggtgagg ggagggataa gtgaggcgtc agtttctttg gtcggtttta
     4201 tgtacctatc ttcttaagta gctgaagctc cggttttgaa ctatgcgctc ggggttggcg
     4261 agtgtgtttt gtgaagtttt ttaggcacct tttgaaatgt aatcatttgg gtcaatatgt
     4321 aattttcagt gttagactag taaattgtcc gctaaattct ggccgttttt ggcttttttg
     4381 ttagacgaag cttgggctgc aggtcgactc tagaaaattg tccgctaaat tctggccgtt
     4441 tttggctttt ttgttagaca ggatcgatcc ccgggtaccg gtcgccacca tggtgagcaa
     4501 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     4561 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     4621 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     4681 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     4741 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     4801 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     4861 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     4921 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     4981 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     5041 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     5101 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     5161 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtacc catacgatgt
     5221 tccagattac gctggcgcgc caatgtttgc agacttagac tatgacatcg aggaggataa
     5281 attaggaatc cccactgtgc ccgggaaggt caccctgcag aaggatgctc agaacctgat
     5341 tggcatcagc atcggaggag gggcccagta ctgtccttgt ctctacattg tccaggtatt
     5401 tgataacacg cctgcagcct tggacggcac tgtggcagct ggtgatgaga tcactggggt
     5461 caatggccga tcaatcaaag gaaaaaccaa ggtagaggtg gcgaagatga ttcaggaggt
     5521 gaagggggag gtgaccatcc attacaacaa gctgcaggcg gaccctaagc agggcatgtc
     5581 cctggacatt gtgttgaaga aagtcaagca ccggctggta gaaaacatga gttcaggcac
     5641 agctgatgcc ctgggtctaa gccgtgccat cctgtgtaat gatgggttgg taaagcggct
     5701 ggaggagctg gagcgcactg ccgagctgta caaaggaatg acggagcaca ccaagaatct
     5761 cctacgggcc ttttatgagc tgtcacagac tcaccgggct tttggggacg tgttctctgt
     5821 gattggggtg cgggagccgc agccagcagc aagtgaagca tttgtgaagt ttgctgacgc
     5881 acaccgcagc attgagaagt tcggcattcg gctactgaaa accatcaaac cgatgctgac
     5941 ggatctgaac acgtacctca ataaggccat ccctgataca cgcctcacca tcaagaagta
     6001 cctggatgtg aagtttgagt acttgtcata ctgtctgaag gtgaaggaga tggacgacga
     6061 ggagtacagc tgcattgccc taggagagcc gctataccga gtgagcacag gcaactatga
     6121 gtataggtta atcctgcgct gccgccagga ggctcgagcc cgcttctccc agatgcgcaa
     6181 ggatgtgctg gagaagatgg agctgctgga ccagaagcac gtccaggaca tcgtgttcca
     6241 gctgcagcgc ttcgtgtcta ccatgtccaa gtactataat gactgctatg cggtgctgcg
     6301 ggacgccgac gtcttcccca tcgaggtgga cctggcacac accactctag cctacggccc
     6361 taaccagggc ggctttaccg atggcgaaga tgaggaggag gaggaggagg acggggcagc
     6421 tagggaagtg tccaaggacg cacgtggggc cactgggcct acagacaagg gcagaagctg
     6481 gtgtgactcc tgacggtccg gtacaagtaa agcggccgcg actctagaat tcgatatcaa
     6541 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     6601 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     6661 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     6721 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     6781 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     6841 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     6901 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     6961 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     7021 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     7081 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
     7141 tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta gcaatacagc
     7201 agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc
     7261 agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     7321 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat
     7381 ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga actacacacc
     7441 agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     7501 gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag
     7561 cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct
     7621 agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga
     7681 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     7741 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     7801 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc
     7861 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     7921 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     7981 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     8041 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     8101 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     8161 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     8221 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     8281 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     8341 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     8401 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     8461 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     8521 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     8581 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     8641 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     8701 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     8761 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     8821 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     8881 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     8941 cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata
     9001 gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg
     9061 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc
     9121 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc
     9181 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc
     9241 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc
     9301 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac
     9361 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat
     9421 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     9481 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     9541 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     9601 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     9661 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     9721 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     9781 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     9841 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     9901 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     9961 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
    10021 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
    10081 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
    10141 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
    10201 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
    10261 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
    10321 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
    10381 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
    10441 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
    10501 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
    10561 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
    10621 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    10681 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
    10741 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
    10801 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
    10861 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
    10921 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
    10981 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    11041 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    11101 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    11161 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    11221 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    11281 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    11341 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    11401 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    11461 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    11521 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    11581 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    11641 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    11701 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    11761 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    11821 ggggttccgc gcacatttcc ccgaaaagtg ccacctgac
//
