LOCUS       sequence-145-exp        3646 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1997
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L3467
FEATURES             Location/Qualifiers
     source          1..3646
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(128..156)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     904..924
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(1035..1052)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1035..1051)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1044..1066)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1260..1279)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1379..1401
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1439..1457)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1525..1629
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1630..1698,1699..2490)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1848..1867)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2661..3249
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3150..3169
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3403..3420
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3537..3558
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3573..3578,3579..3596,3597..3603)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3611..3627
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3616..3638
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(3635..3646,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(3635..3646,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccaag cttcagggcc cgagctcgac gtcccgcgga ttaatataat
       61 tatgagtcga caaggaattc aagtaccggt agaaaaaatg agtaaaggag aagaactttt
      121 cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc
      181 tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg
      241 cactactgga aaactacctg ttccatgggt aagtttaaac atatatatac taactaaccc
      301 tgattattta aattttcagc caacacttgt cactactctc acttatggtg ttcaatgctt
      361 ctcgagatac ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg
      421 ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtaagtt
      481 taaacagttc ggtactaact aaccatacat atttaaattt tcaggtgctg aagtcaagtt
      541 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg
      601 aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc
      661 agacaaacaa aagaatggaa tcaaagttgt aagtttaaac atgattttac taactaacta
      721 atctgattta aattttcaga acttcaaaat tagacacaac attgaagatg gaagcgttca
      781 actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga
      841 caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca
      901 catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg acgaactata
      961 caaaagccca caagcttcag ggcccgagct cgacgtcccg cggattaata taattatgag
     1021 tcgacaagga attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     1081 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     1141 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg
     1201 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
     1261 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     1321 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     1381 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
     1441 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     1501 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     1561 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     1621 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     1681 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     1741 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     1801 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     1861 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     1921 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     1981 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     2041 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     2101 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     2161 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     2221 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     2281 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     2341 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     2401 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     2461 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     2521 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     2581 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     2641 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     2701 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     2761 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     2821 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     2881 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     2941 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     3001 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     3061 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     3121 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     3181 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     3241 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     3301 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     3361 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     3421 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     3481 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     3541 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     3601 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagct
//
