LOCUS       sequence-145561-         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human codon-optimized dCas9-DNMT3A fusion with 
            T2A-PuroR (v2) for targeted DNA methylation in mammalian cells; 
            cloning backbone for sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Vojta A, Dobrinic P, Tadic V, Bockor L, Korac P, Julg B, Klasic M, 
            Zoldos V
  TITLE     Repurposing the CRISPR-Cas9 system for targeted DNA methylation.
  JOURNAL   Nucleic Acids Res. 2016 Mar 11. pii: gkw159.
  PUBMED    26969735
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Mar 11. pii: gkw159."
COMMENT     Sequence Label: pdCas9-DNMT3A-PuroR_v2
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     123..142
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        219..294
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        391..676
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata
       61 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa
      121 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct
      181 tgtggaaagg acgaaacacc gggtcttcga gaagacctgt tttagagcta gaaatagcaa
      241 gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt
      301 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgtttttagc gcgtgcgcca
      361 attctgcaga caaatggctc tagaggtacc cgttacataa cttacggtaa atggcccgcc
      421 tggctgaccg cccaacgacc cccgcccatt gacgtcaata gtaacgccaa tagggacttt
      481 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      541 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      601 ttgtgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      661 catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc
      721 cccctcccca cccccaattt tgtatttatt tattttttaa ttatttt
//
