LOCUS       sequence-145565-         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  shRNA(1) against CDK6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Acevedo M, Vernier M, Mignacca L, Lessard F, Huot G, Moiseeva O, 
            Bourdeau V, Ferbeyre G
  TITLE     A CDK4/6-Dependent Epigenetic Mechanism Protects Cancer Cells from 
            PML-induced Senescence.
  JOURNAL   Cancer Res. 2016 Jun 1;76(11):3252-64. doi: 
            10.1158/0008-5472.CAN-15-2347. Epub 2016 Mar 29.
  PUBMED    27206849
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/0008-5472.CAN-15-2347"; journalName: "Cancer Res"; date: 
            "2016-06-1- 1"; volume: "76"; issue: "11"; pages: "3252-64"
COMMENT     Sequence Label: shCDK6(1)
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           61..182
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           210..224
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           252..378
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(432..455)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     829..848
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 ctcactcctt ctctaggcgc cggaattaga tctctcgact agggataaca gggtaattgt
       61 ttgaatgagg cttcagtact ttacagaatc gttgcctgca catcttggaa acacttgctg
      121 ggattacttc ttcaggttaa cccaacagaa ggctcgagaa ggtatattgc tgttgacagt
      181 gagcgtagtc ttgctccagt ccagctatag tgaagccaca gatgtatagc tggactggag
      241 caagactttg cctactgcct cggaattcaa ggggctactt taggagcaat tatcttgttt
      301 actaaaactg aataccttgc tatctctttg atacattttt acaaagctga attaaaatgg
      361 tataaattaa atcacttttt tggatccaat tctaccgggt aggggaggcg cttttcccaa
      421 ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc
      481 tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg
      541 gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc
      601 tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg
      661 acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt
      721 gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg
      781 gcgggctcaa gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc
      841 ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctc
//
