LOCUS       sequence-145567-         585 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  shRNA(1) against CDK6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Acevedo M, Vernier M, Mignacca L, Lessard F, Huot G, Moiseeva O, 
            Bourdeau V, Ferbeyre G
  TITLE     A CDK4/6-Dependent Epigenetic Mechanism Protects Cancer Cells from 
            PML-induced Senescence.
  JOURNAL   Cancer Res. 2016 Jun 1;76(11):3252-64. doi: 
            10.1158/0008-5472.CAN-15-2347. Epub 2016 Mar 29.
  PUBMED    27206849
REFERENCE   2  (bases 1 to 585)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/0008-5472.CAN-15-2347"; journalName: "Cancer Res"; date: 
            "2016-06-1- 1"; volume: "76"; issue: "11"; pages: "3252-64"
COMMENT     Sequence Label: shCDK6(1)
FEATURES             Location/Qualifiers
     source          1..585
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           complement(27..153)
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(181..195)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(223..344)
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(400..416)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(432..454)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 ccctacccgg tagaattgga tccaaaaaag tgatttaatt tataccattt taattcagct
       61 ttgtaaaaat gtatcaaaga gatagcaagg tattcagttt tagtaaacaa gataattgct
      121 cctaaagtag ccccttgaat tccgaggcag taggcaaagt cttgctccag tccagctata
      181 catctgtggc ttcactatag ctggactgga gcaagactac gctcactgtc aacagcaata
      241 taccttctcg agccttctgt tgggttaacc tgaagaagta atcccagcaa gtgtttccaa
      301 gatgtgcagg caacgattct gtaaagtact gaagcctcat tcaaacaatt accctgttat
      361 ccctagtcga gagatctaat tccggcgcct agagaaggag tgagggctgg ataaagggag
      421 gatcgaggcg gggtcgaacg aggaggttca agggggagag acggggcgga tggaggaaga
      481 ggaggcggag gcttagggtg tacaaagggc ttgacccagg gaggggggtc aaaagccaag
      541 gcttcccagg tcacgatgta ggggacctgg tctgggtgtc catgc
//
