LOCUS       sequence-145577-         578 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive Luciferase expression in mammalian cells along with 
            puromycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Giannou AD, Marazioti A, Kanellakis NI, Giopanou I, Lilis I, Zazara 
            DE, Ntaliarda G, Kati D, Armenis V, Giotopoulou GA, Krontira AC, 
            Lianou M, Agalioti T, Vreka M, Papageorgopoulou M, Fouzas S, 
            Kardamakis D, Psallidas I, Spella M, Stathopoulos GT
  TITLE     NRAS destines tumor cells to the lungs.
  JOURNAL   EMBO Mol Med. 2017 May;9(5):672-686. doi: 10.15252/emmm.201606978.
  PUBMED    28341702
REFERENCE   2  (bases 1 to 578)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.15252/emmm"; 
            journalName: "EMBO Mol Med"; date: "2017-05"; volume: "9"; issue: 
            "5"; pages: "672-686"
COMMENT     Sequence Label: Cag.Luc.puro
FEATURES             Location/Qualifiers
     source          1..578
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     81..99
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     regulatory      162..171
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(239..261)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 ctcagcgtaa gtgatgtcca cctcgatatg tgcatctgta aaagcaattg ttccaggaac
       61 cagggcgtat ctcttcatag ccttatgcag ttgctctcca gcggttccat cttccagcgg
      121 atagaatggc gccgggcctt tctttatgtt tttggcgtct tccatggtgg ctttaccaac
      181 agtaccggaa tgccaagctt acttagatcg cagatctcga gcccgggcta gagccgccgg
      241 tcacacgcca gaagccgaac cccgccctgc cccgtccccc ccgaaggcag ccgtcccccc
      301 gcggacagcc ccgaggctgg agagggagaa ggggacggcg gcgcggcgac gcacgaaggc
      361 cctccccgcc catttccttc ctgccggcgc cgcaccgctt cgccccgcgc ccgctagagg
      421 gggtgcggcg gcgcctccca gatttcggct ccgcacagat ttgggacaaa ggaagtccct
      481 gcgccctctc gcacgattac cataaaaggc aatggctgcg gctcgccgcg cctcgacagc
      541 cgccggcgct ccgggggccg ccgcgcccct cccccgag
//
