LOCUS       sequence-145587-         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Light-induced nuclear export switch for microscopy in mammalian 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Yumerefendi H, Lerner AM, Zimmerman SP, Hahn K, Bear JE, Strahl BD, 
            Kuhlman B
  TITLE     Light-induced nuclear export reveals rapid dynamics of epigenetic 
            modifications.
  JOURNAL   Nat Chem Biol. 2016 Jun;12(6):399-401. doi: 10.1038/nchembio.2068. 
            Epub 2016 Apr 18.
  PUBMED    27089030
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.2068"; journalName: "Nat Chem Biol"; date: 
            "2016-06"; volume: "12"; issue: "6"; pages: "399-401"
COMMENT     Sequence Label: pTriEx-mCherry-LINXa3
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    21..45
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        61..170
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             185..892
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(330..348)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 aattatcgac tcactatagg ggaattgtga gcggataaca attccccgga gttaatccgg
       61 gacctttaat tcaacccaac acaatatatt atagttaaat aagaattatt atcaaatcat
      121 ttgtatatta attaaaatac tatactgtaa attacatttt atttacaatc aaaggagata
      181 taccatggtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt
      241 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
      301 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct
      361 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa
      421 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga
      481 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
      541 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc
      601 tgtaatgcag aagaagacta tgggctggga ggcctcctcc gagcggatgt accccgagga
      661 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga
      721 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa
      781 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta
      841 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca aggg
//
