LOCUS       sequence-145588-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Light-induced nuclear export switch for microscopy in mammalian 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Yumerefendi H, Lerner AM, Zimmerman SP, Hahn K, Bear JE, Strahl BD, 
            Kuhlman B
  TITLE     Light-induced nuclear export reveals rapid dynamics of epigenetic 
            modifications.
  JOURNAL   Nat Chem Biol. 2016 Jun;12(6):399-401. doi: 10.1038/nchembio.2068. 
            Epub 2016 Apr 18.
  PUBMED    27089030
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.2068"; journalName: "Nat Chem Biol"; date: 
            "2016-06"; volume: "12"; issue: "6"; pages: "399-401"
COMMENT     Sequence Label: pTriEx-mCherry-LINXa3
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(41..64)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(71..103)
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             complement(158..586)
                     /codon_start=1
                     /gene="NPH1 from Avena sativa"
                     /product="light, oxygen, or voltage domain from phototropin
                     1 of Avena sativa (Herrou and Crosson, 2011)"
                     /label=LOV2
                     /note="photoswitch that undergoes a light-inducible 
                     conformational change"
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQ
                     YFIGVQLDGTEHVRDAAEREGVMLIKKTAENIDEAAKEL"
ORIGIN
        1 accttctgat aggcagcctg cacctgaggt taatcactta gtgatggtga tggtgatggt
       61 ggtgctcgag atcctcgggg tcttccgggg cgagttctgg ctggcttgca cgtgtataca
      121 gctgtgcggc cgcaagcttt tacagatcca gcccggccag ctcttttgcc gcttcatcaa
      181 tattttctgc agttttctta atcagcatga ctccctctct ctcggcagca tctcggacat
      241 gctcagttcc atccaactga accccaataa agtactggac atctcccttc tgatctcgca
      301 taggctgcaa gtgaaagagg ttccagaact ttttaccact ctttgtataa ttaatcagct
      361 gaacagtgac ctctgtttgg ttatctatgg catctctaat ttttctcact gtcgcgcgat
      421 cagtttcagg accttgtaga aacctgcagt ttcttcccaa aatttcttca cggctatatt
      481 ctgtcaactg caagaaacta tcggacgcga atataatggg attatctggc aatcttgggt
      541 cagtaatgac aaagttcttc tcaatacgtt caagtgtagt agccaaggat ccacttccag
      601 aaccgtccag cttcgccctc ttggcggccg gggtaccctt gtacagctcg tccatgccgc
      661 cggtggagtg gcggccctcg gcgcgttcgt actgttccac gatggtgtag tcctcgttgt
      721 gggaggtgat gtccaacttg atgttgacgt tgtaggcgcc gggcagctgc acgggcttct
      781 tggccttgta ggtggtcttg acctcagcgt cgtagtggcc gccgtccttc agcttcagcc
      841 tctgcttgat
//
