LOCUS       sequence-145602-         725 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gene targeting vector for the mouse Rosa26 locus, including a loxP 
            flanked stop cassette and GFP, for gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Chu VT, Weber T, Graf R, Sommermann T, Petsch K, Sack U, Volchkov P,
            Rajewsky K, Kuhn R
  TITLE     Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in 
            C57BL/6 zygotes.
  JOURNAL   BMC Biotechnol. 2016 Jan 16;16(1):4. doi: 10.1186/s12896-016-0234-4.
  PUBMED    26772810
REFERENCE   2  (bases 1 to 725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-01-16"; volume: "16(1):4. doi"; pages: " 
            10.1186/s12896-016-0234-4"
COMMENT     Sequence Label: pR26 GFP Dest
FEATURES             Location/Qualifiers
     source          1..725
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     71..94
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ggcggcggcg cctaccggtg gatgtggaat gtgtgcgagg ccagaggcca cttgtgtagc
       61 gccaagtgcc cagcggggct gctaaagcgc atgctccaga ctgccttggg aaaagcgcct
      121 cccctacccg gtagaattcg atatcaagct tatcgatacc gtcgacggta tcgataagct
      181 agatgtcctg aaatatttct atcagaacaa ggtagtataa agctggtagg tatacaaaac
      241 gctagactag tttctatccc tgacccttaa tctgctagta tatccgtagg aagttgctta
      301 agtgccacta gtaccaacag cctctgttag agtgaacagt agattttaat gaagggccaa
      361 taaagaccac ttaagactac tgactgaatt aaaaatccac ccagtggagt tagtattggt
      421 tatagttata taacaaaata tccaacttag ccaggcgtgg tggtgcacgc ctttaatccc
      481 ggcactcagg aggcagaggc aggcggattt ctgagttcga ggccagcctg gtctacaaag
      541 tgagttccag gacagccagg gctatacaga gaaaccctgt ctcgaaaaac caaaaaaaaa
      601 aaaaaaaagg aaaaaaaata tggcttgaaa acatgctatt gttgtcaaaa ggagatccaa
      661 acaagtattt ctttgaacaa aaattatgta gaaaacaggg tgataaagaa caagggcttt
      721 ttaaa
//
