LOCUS       sequence-145625-         283 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses high specificity SpCas9. Px330-like plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Slaymaker IM, Gao L, Zetsche B, Scott DA, Yan WX, Zhang F
  TITLE     Rationally engineered Cas9 nucleases with improved specificity.
  JOURNAL   Science. 2015 Dec 1. pii: aad5227.
  PUBMED    26628643
REFERENCE   2  (bases 1 to 283)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2015 Dec 1. pii: aad5227."
COMMENT     Sequence Label: eSpCas9(1.1)
FEATURES             Location/Qualifiers
     source          1..283
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
       61 tgacggtaaa tggcccgcct ggcattgtgc ccagtacatg accttatggg actttcctac
      121 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt
      181 ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt
      241 ttaattattt tgtgcagcga tgggggcggg gggggggggg ggg
//
