LOCUS       sequence-145626-         714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RPU standard plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Nielsen AA, Der BS, Shin J, Vaidyanathan P, Paralanov V, Strychalski
            EA, Ross D, Densmore D, Voigt CA
  TITLE     Genetic circuit design automation.
  JOURNAL   Science. 2016 Apr 1;352(6281):aac7341. doi: 10.1126/science.aac7341.
  PUBMED    27034378
REFERENCE   2  (bases 1 to 714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2016-04-1"; volume: "352(6281):aac7341. doi"; pages: " 
            10.1126/science.aac7341"
COMMENT     Sequence Label: pAN1717
FEATURES             Location/Qualifiers
     source          1..714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(172..191)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 agtaactcgg taccaaattc cagaaaagag gcctcccgaa aggggggcct tttttcgttt
       61 tggtccaatg gcggcgcgcc atcgaatggc gcaaaacctt tcgcggtatg gcatgatagc
      121 gcccggaaga gagtcaattc agggtggtga atatgaaacc agtaacgtta tacgatgtcg
      181 cagagtatgc cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg
      241 tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca
      301 accgcgtggc acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca
      361 gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac
      421 tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg
      481 cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg
      541 accaggatgc cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg
      601 tctctgacca gacacccatc aacagtatta ttttctccca tgaggacggt acgcgactgg
      661 gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcc
//
