LOCUS       sequence-145630-         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Toehold switch sensor to detect Zika Virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Pardee K, Green AA, Takahashi MK, Braff D, Lambert G, Lee JW, 
            Ferrante T, Ma D, Donghia N, Fan M, Daringer NM, Bosch I, Dudley DM,
            O'Connor DH, Gehrke L, Collins JJ
  TITLE     Rapid, Low-Cost Detection of Zika Virus Using Programmable 
            Biomolecular Components.
  JOURNAL   Cell. 2016 May 6. pii: S0092-8674(16)30505-0. doi: 
            10.1016/j.cell.2016.04.059.
  PUBMED    27160350
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2016 
            May 6. pii: S0092-8674(16)30505-0. doi: 10.1016/j.cell.2016.04.059."
COMMENT     Sequence Label: ZIKV_Sensor_27B_LacZ
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             73..246
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEXART
                     DRPSQQLRSLNGE"
     primer_bind     complement(93..110)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(93..109)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(102..124)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tcggacttta gaacagagga gataaagatg gacacaggac acaacctggc ggcagcgcaa
       61 aagatgcgta aaatgaccat gattacggat tcactggccg tcgttttaca acgtcgtgac
      121 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
      181 tggcgtaata gcgaanaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
      241 ggcgaatggc gctttgcctg gtttccggca ccagaagcgg tgccggaaag ctggctggag
      301 tgcgatcttc ctgaggccga tactgtcgtc gtcccctcaa actggcanat gcacggttac
      361 natgcgccca tctacaccaa cgtgacctat cccattacgg tcaatccgcc gtttgttccc
      421 acggagaatc cgacgggttg ttactcgctc acatttaatg ttgatgaaag ctggctacag
      481 gaaggccaga cgcgaattat ttttgatggc gttaactcgg cgtttcatct gtggtgcaac
      541 gggcgctggg tcggttacgg ccaggacagt cgtttgccgt ctgaatttga cctgagcgca
      601 tttttacgcg ccggagaaaa ccgcctcgcg gtgatggtgc tgcgctggag tgacggcagt
      661 tatctggaag atcaggatat gtggcggatg agcggcattt tccgtgacgt ctcgttgctg
      721 cataaaccga ctacacaaat cagcgatttc catgttgcca ctcgctttaa tgatgatttc
      781 agccgcgctg tactggaggc tgaa
//
