LOCUS       sequence-145633-         688 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Portion of Zika Virus genome (KU312312: 1348-1494) containing sensor
            27B trigger sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Pardee K, Green AA, Takahashi MK, Braff D, Lambert G, Lee JW, 
            Ferrante T, Ma D, Donghia N, Fan M, Daringer NM, Bosch I, Dudley DM,
            O'Connor DH, Gehrke L, Collins JJ
  TITLE     Rapid, Low-Cost Detection of Zika Virus Using Programmable 
            Biomolecular Components.
  JOURNAL   Cell. 2016 May 6. pii: S0092-8674(16)30505-0. doi: 
            10.1016/j.cell.2016.04.059.
  PUBMED    27160350
REFERENCE   2  (bases 1 to 688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2016 
            May 6. pii: S0092-8674(16)30505-0. doi: 10.1016/j.cell.2016.04.059."
COMMENT     Sequence Label: ZIKV_Trigger_27B
FEATURES             Location/Qualifiers
     source          1..688
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      117..163
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(338..343,344..360,361..366))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(393..411)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        479..583
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggacatgaaa ctgatgagaa tagagcgaaa gttgagataa cgcccaattc accaagagcc
       61 gaagccaccc tgggggggtt tggaagccta ggacttgatt gtgaaccgag gacaggtagc
      121 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat
      181 atccggatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca
      241 tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc
      301 tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc
      361 tgtcaaacat gagaattctt gaanacgaaa gggcctcgtg atacgcctat ttttataggt
      421 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
      481 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
      541 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
      601 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
      661 aacgctggtg aaagtaaaag atgctgaa
//
