LOCUS       sequence-145639-         728 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Portion of Zika Virus genome (KU312312: 7166-7299) containing sensor
            32B trigger sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Pardee K, Green AA, Takahashi MK, Braff D, Lambert G, Lee JW, 
            Ferrante T, Ma D, Donghia N, Fan M, Daringer NM, Bosch I, Dudley DM,
            O'Connor DH, Gehrke L, Collins JJ
  TITLE     Rapid, Low-Cost Detection of Zika Virus Using Programmable 
            Biomolecular Components.
  JOURNAL   Cell. 2016 May 6. pii: S0092-8674(16)30505-0. doi: 
            10.1016/j.cell.2016.04.059.
  PUBMED    27160350
REFERENCE   2  (bases 1 to 728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2016 
            May 6. pii: S0092-8674(16)30505-0. doi: 10.1016/j.cell.2016.04.059."
COMMENT     Sequence Label: ZIKV_Trigger_32B
FEATURES             Location/Qualifiers
     source          1..728
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      104..150
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(325..330,331..347,348..353))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(380..398)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        466..570
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tggcgcacta catgtacttg atcccagggc tgcaggcagc agctgcgcgt gctgcccaga
       61 agagaacggc agctggcatc atgaagaacc ctgttgtgga tggtagcata accccttggg
      121 gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatccc
      181 gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg
      241 tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc
      301 aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag
      361 aattcttgaa nacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
      421 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
      481 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
      541 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
      601 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
      661 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
      721 agcggtaa
//
