LOCUS       sequence-145640-         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Portion of Zika Virus genome (KU312312: 7166-7299) containing sensor
            32B trigger sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Pardee K, Green AA, Takahashi MK, Braff D, Lambert G, Lee JW, 
            Ferrante T, Ma D, Donghia N, Fan M, Daringer NM, Bosch I, Dudley DM,
            O'Connor DH, Gehrke L, Collins JJ
  TITLE     Rapid, Low-Cost Detection of Zika Virus Using Programmable 
            Biomolecular Components.
  JOURNAL   Cell. 2016 May 6. pii: S0092-8674(16)30505-0. doi: 
            10.1016/j.cell.2016.04.059.
  PUBMED    27160350
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2016 
            May 6. pii: S0092-8674(16)30505-0. doi: 10.1016/j.cell.2016.04.059."
COMMENT     Sequence Label: ZIKV_Trigger_32B
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(191..295)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     363..381
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(408..413,414..430,431..436)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     terminator      complement(611..657)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(792..811)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(793..811)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(915..934)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
       61 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
      121 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
      181 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
      241 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
      301 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
      361 aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt ctcatgtttg acagcttatc
      421 atcgataagc tttaatgcgg tagtttatca cagttaaatt gctaacgcag tcaggcaccg
      481 tgtatgaaat ctaacaatgc gctcatcgtc atcctcggca ccgtcaccct ggatgctgta
      541 ggcataggct tggttatgcc ggtactgccg ggcctcttgc gggatatccg gatatagttc
      601 ctcctttcag caaaaaaccc ctcaagaccc gtttagaggc cccaaggggt tatgctacca
      661 tccacaacag ggttcttcat gatgccagct gccgttctct tctgggcagc acgcgcagct
      721 gctgcctgca gccctgggat caagtacatg tagtgcgcca cgagcaaaat gatggccact
      781 attagggtca gccctatagt gagtcgtatt agcgcaacga aagccagata gcccgtacac
      841 gttcgagatc tcgatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg
      901 tgcggttgct ggcgcctata tcgccgacat caccgatggg ga
//
