LOCUS       sequence-145643-         629 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Portion of Zika Virus genome (KU312312: 1248-1594) containing sensor
            27B trigger sequence and NASBA amplification sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Pardee K, Green AA, Takahashi MK, Braff D, Lambert G, Lee JW, 
            Ferrante T, Ma D, Donghia N, Fan M, Daringer NM, Bosch I, Dudley DM,
            O'Connor DH, Gehrke L, Collins JJ
  TITLE     Rapid, Low-Cost Detection of Zika Virus Using Programmable 
            Biomolecular Components.
  JOURNAL   Cell. 2016 May 6. pii: S0092-8674(16)30505-0. doi: 
            10.1016/j.cell.2016.04.059.
  PUBMED    27160350
REFERENCE   2  (bases 1 to 629)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2016 
            May 6. pii: S0092-8674(16)30505-0. doi: 10.1016/j.cell.2016.04.059."
COMMENT     Sequence Label: ZIKV_NASBA_27B
FEATURES             Location/Qualifiers
     source          1..629
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      288..334
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(509..514,515..531,532..537))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(564..582)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 agaatctgga gtaccggata atgctgtcag ttcatggctc ccagcacagt gggatgatcg
       61 ttaatgacac aggacatgaa actgatgaga atagagcgaa agttgagata acgcccaatt
      121 caccaagagc cgaagccacc ctgggggggt ttggaagcct aggacttgat tgtgaaccga
      181 ggacaggcct tgacttttca natttgtatt acttgactat gaataacaag cactggctgg
      241 ttcacaagga gtggttccac gacattccat taccttggca cgctgggtag cataacccct
      301 tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata
      361 tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg
      421 acggtgccga ggatgacnat gagcgcattg ttagatttca tacacggtgc ctgactgcgt
      481 tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca
      541 tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
      601 gataataatg gtttcttaga cgtcaggtg
//
