LOCUS       sequence-145645-         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Portion of Zika Virus genome (KU312312: 7066-7399) containing sensor
            32B trigger sequence and NASBA amplification sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Pardee K, Green AA, Takahashi MK, Braff D, Lambert G, Lee JW, 
            Ferrante T, Ma D, Donghia N, Fan M, Daringer NM, Bosch I, Dudley DM,
            O'Connor DH, Gehrke L, Collins JJ
  TITLE     Rapid, Low-Cost Detection of Zika Virus Using Programmable 
            Biomolecular Components.
  JOURNAL   Cell. 2016 May 6. pii: S0092-8674(16)30505-0. doi: 
            10.1016/j.cell.2016.04.059.
  PUBMED    27160350
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2016 
            May 6. pii: S0092-8674(16)30505-0. doi: 10.1016/j.cell.2016.04.059."
COMMENT     Sequence Label: ZIKV_NASBA_32B
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      302..348
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(523..528,529..545,546..551))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(578..596)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        664..768
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccattctac gcatgggact ttggagtccc gctgctaatg ataggttgct actcacaatt
       61 aacacccctg accctaatag tggccatcat tttgctcgtg gcgcactaca tgtacttgat
      121 cccagggctg caggcagcag ctgcgcgtgc tgcccagaan agaacggcag ctggcatcat
      181 gaanaaccct gttgtggatg gaatagtggt gactgacatt gacacaatga caattgaccc
      241 ccaagtggag aaaaagatgg gacaggtgct actcatagca gtagccgtct ccagcgccat
      301 atagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga
      361 actatatccg gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta
      421 cagcatccag ggtgacggtg ccnaggatga cnatgagcgc attgttagat ttcatacacg
      481 gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga
      541 taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
      601 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
      661 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
      721 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
      781 catttccgtg tcgccc
//
