LOCUS       sequence-145646-         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Portion of Zika Virus genome (KU312312: 7066-7399) containing sensor
            32B trigger sequence and NASBA amplification sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Pardee K, Green AA, Takahashi MK, Braff D, Lambert G, Lee JW, 
            Ferrante T, Ma D, Donghia N, Fan M, Daringer NM, Bosch I, Dudley DM,
            O'Connor DH, Gehrke L, Collins JJ
  TITLE     Rapid, Low-Cost Detection of Zika Virus Using Programmable 
            Biomolecular Components.
  JOURNAL   Cell. 2016 May 6. pii: S0092-8674(16)30505-0. doi: 
            10.1016/j.cell.2016.04.059.
  PUBMED    27160350
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2016 
            May 6. pii: S0092-8674(16)30505-0. doi: 10.1016/j.cell.2016.04.059."
COMMENT     Sequence Label: ZIKV_NASBA_32B
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(174..278)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     346..364
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(391..396,397..413,414..419)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     terminator      complement(594..640)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
       61 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
      121 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
      181 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
      241 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
      301 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
      361 aggccctttc gtcttcaaga attctcatgt ttgacagctt atcatcgata agctttaatg
      421 cggtagttta tcacagttaa attgctaacg cagtcaggca ccgtgtatga aatctaacaa
      481 tgcgctcatc gtcatcctcg gcaccgtcac cctggatgct gtaggcatag gcttggttat
      541 gccggtactg ccgggcctct tgcgggatat ccggatatag ttcctccttt cagcaaaaaa
      601 cccctcaaga cccgtttaga ggccccaagg ggttatgcta tatggcgctg gagacggcta
      661 ctgctatgag tagcacctgt cccatctttt tctccacttg ggggtcaatt gtcattgtgt
      721 caatgtcagt caccactatt ccatccacaa cagggttctt catgatgcca gctgccgttc
      781 tcttctgggc agcacgcgca gctgctgcct gcagccctgg gatcaagtac atgtagtgcg
      841 ccacgagcaa aatgatggcc actattaggg tcaggggtgt taattgtgag tagca
//
