LOCUS       sequence-145649-         448 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gRNA_A to knockout human AMPK alpha 2 using Cas9n..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Toyama EQ, Herzig S, Courchet J, Lewis TL Jr, Loson OC, Hellberg K, 
            Young NP, Chen H, Polleux F, Chan DC, Shaw RJ
  TITLE     Metabolism. AMP-activated protein kinase mediates mitochondrial 
            fission in response to energy stress.
  JOURNAL   Science. 2016 Jan 15;351(6270):275-81. doi: 10.1126/science.aab4138.
  PUBMED    26816379
REFERENCE   2  (bases 1 to 448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2016-01-15- 15"; volume: "351"; 
            issue: "6270"; pages: "275-81"
COMMENT     Sequence Label: pX462-hPRKAA2-gRNA_A
FEATURES             Location/Qualifiers
     source          1..448
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             18..41
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             48..68
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 acaaggatca tgatattgat tacaaagacg atgacgataa gatggcccca aagaagaagc
       61 ggaaggtcgg tatccacgga gtcccagcag ccgacaagaa gtacagcatc ggcctggcca
      121 tcggcaccaa ctctgtgggc tgggccgtga tcaccgacga gtacaaggtg cccagcaaga
      181 aattcaaggt gctgggcaac accgaccggc acagcatcaa gaagaacctg atcggagccc
      241 tgctgttcga cagcggcgaa acagccgagg ccacccggct gaagagaacc gccagaagaa
      301 gatacaccag acggaagaac cggatctgct atctgcaaga gatcttcagc aacgagatgg
      361 ccaaggtgga cgacagcttc ttccacagac tggaagagtc cttcctggtg gaagaggata
      421 agaagcacga gcggcacccc atcttcgg
//
