LOCUS       sequence-145657-         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AKAP13
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..43
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..88
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg gtgtagatct
       61 gggtaccgag aacctgtact tccaatccat gtccaaacag ctggaagcag agtcttggag
      121 tcggataata gacagcaagt ttctaaaaca gcaaaagaaa gatgtggtca aacggcaaga
      181 agtaatatat gagttgatgc agacagagtt tcatcatgtc cgcactctca agatcatgag
      241 tggtgtgtac agccagggga tgatggcgga tctgcttttt gagcagcaga tggtagaaaa
      301 gctgttcccc tgtttggatg agctgatcag tatccatagc caattcttcc agaggattct
      361 ggagcggaag aaggagtctc tggtggataa aagtgaaaag aactttctca tcaagaggat
      421 aggggatgtg cttgtaaatc agttttcagg tgagaatgca gaacgtttaa agaagacata
      481 tggcaagttt tgtgggcaac ataaccagtc tgtaaactac ttcaaagacc tttatgccaa
      541 ggataagcgt tttcaagcct ttgtaaagaa gaagatgagc agttcagttg ttagaaggct
      601 tggaattcca gagtgcatat tgcttgtaac tcagcggatt accaagtacc cagttttatt
      661 ccaaagaata ttgcagtgta ccaaagacaa tgaagtggag caggaagatc tagcacagtc
      721 cttgagcctg gtgaaggatg tgattggagc tgtagacagc aaagtggcaa gttatgaaa
//
