LOCUS       sequence-145658-        1046 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor vector for 3' FLAG tag of human ZNF3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1046)
  TITLE     Mendenhall CRISPR tagging plasmids
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFETCh_ZNF3
FEATURES             Location/Qualifiers
     source          1..1046
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(53..109)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(125..148)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(152..175)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(179..202)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cggccggaga acctgcgtgc aatccatctt gttcaatcat gcgaaacgat ccaggtccag
       61 ggttctcctc cacgtctcca gcctgcttca gcaggctgaa gttagtagct ccgcttcctg
      121 aaaccttatc gtcgtcatcc ttgtaatctc ccttatcgtc gtcatccttg taatcgccct
      181 tatcgtcgtc atccttgtaa tctccagaac cacctccgcc ggaacctcct ccgctccccg
      241 tggactctct gatatttaac tcggtcgtaa ctctgaggga gcttttgctc atgccgatcc
      301 cattcaaagg cttctctcca gtgtgaattc tctgatggcg aacaagagcc gagctgtacc
      361 tgaaggcttt cccacactca ctacattcat aggggttctc cccggtgtgg attctttgat
      421 gctgaataag gcctgaactg taggtaaact ttcctccaca ttccatacat tcgtagggct
      481 tctctccagt gtggattctc tggtgctgat agaggtgtga gctctggctg aaggctttcc
      541 cacattcatt acattcatag ggtttttctc cagtgtggat tctctgatgg tgaataaggg
      601 ttgagctcct gctgaaggcc ttcccacatt cattgcaggc gtagggtttc tcaccagtgt
      661 ggattctctg atggtgggtg agggtggagc tccagctgaa ggtcttccca cactcattac
      721 attcataggg tttctccccc gtgtggatcc tccgatgcag aatgagggca gagctacagc
      781 tgaaggtttt cccacaatca ctacattcat aaggtttttc cccagtgtgg attctctgat
      841 gctgaataag gtgtgagctc tggctgaagg ccttcccaca ctcattacat tcatagggct
      901 tttccccagt gtggattctc tgatgttgaa taaggtctga agttcgatta aagctcttgc
      961 tacattcatc acacttatgg ggtctgtctc ccagctgggc tgcaggtcgg ggagaggagt
     1021 gttagtaacc gcgacgctgg tggggg
//
