LOCUS       sequence-145669-         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  vector encoding a fusion protein between PML-IV and ER (estrogen 
            receptor).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Acevedo M, Vernier M, Mignacca L, Lessard F, Huot G, Moiseeva O, 
            Bourdeau V, Ferbeyre G
  TITLE     A CDK4/6-Dependent Epigenetic Mechanism Protects Cancer Cells from 
            PML-induced Senescence.
  JOURNAL   Cancer Res. 2016 Jun 1;76(11):3252-64. doi: 
            10.1158/0008-5472.CAN-15-2347. Epub 2016 Mar 29.
  PUBMED    27206849
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/0008-5472.CAN-15-2347"; journalName: "Cancer Res"; date: 
            "2016-06-1- 1"; volume: "76"; issue: "11"; pages: "3252-64"
COMMENT     Sequence Label: PML-ER-pBabe
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..928)
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     CDS             complement(41..976)
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
ORIGIN
        1 accggggtac cgaattaatt ccgccaggga gctctcagac tgtggcaggg aaaccctctg
       61 cctcccccgt gatgtaatac ttttgcaagg aatgcgatga agtagagccc gcagtggcca
      121 agtggctttg gtccgtctcc tccacggatg cccctccacg gctagtgggc gcatgtaggc
      181 ggtgggcgtc cagcatctcc agcagcaggt catagagggg caccacgttc ttgcacttca
      241 tgctgtacag atgctccatg cctttgttac tcatgtgcct gatgtgggag aggatgagga
      301 ggagctgggc cagccgctgg tgctgctgct gcagggtcag gcctgccttg gccatcaggt
      361 ggatcaaagt gtctgtgatc ttgtccagga ctcggtggat atggtccttc tcttccagag
      421 acttcagggt gctggacaga aatgtgtaca ctccagaatt aagcaaaata atagatttga
      481 ggcacacaaa ctcctctccc tgcagattca tcatgcggaa ccgagatgat gtagccagca
      541 gcatgtcgaa gatctccacc atgccctcta cacattttcc ctggttcctg tccaagagca
      601 agttaggagc aaacagtagc ttcactgggt gctccatgga gcgccagacg agaccaatca
      661 tcaggatctc tagccaggca cattctagaa ggtggacctg atcatggagg gtcaaatcca
      721 caaagcctgg caccctcttc gcccagttga tcatgtgaac cagctccctg tctgccaggt
      781 tggtcagtaa gcccatcatc gaagcttcac tgaagggtct ggtaggatca tactcggaat
      841 agagtatggg gggctcagca tccaacaagg cactgaccat ctggtcggcc gtcagggaca
      901 aggccaggct gttcttctta gagcgtttga tcatgagcgg gcttggccaa aggttggcag
      961 ctctcatgtc tccagcagat ggatcggaat
//
