LOCUS       sequence-145680-         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lenti sgRNA cloning backbone with MS2 loops at tetraloop and 
            stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI 
            sites for insertion of spacer sequences..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham
            MD, Sanjana NE, Zhang F
  TITLE     Genome-scale CRISPR-Cas9 knockout and transcriptional activation 
            screening.
  JOURNAL   Nat Protoc. 2017 Apr;12(4):828-863. doi: 10.1038/nprot.2017.016. 
            Epub 2017 Mar 23.
  PUBMED    28333914
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2017.016"; journalName: "Nat Protoc"; date: 
            "2017-04"; volume: "12"; issue: "4"; pages: "828-863"
COMMENT     Sequence Label: lentiSAMv2
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             18..65
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 ggcgtacggc caccatgaaa aggccggcgg ccacgaaaaa ggccggccag gcaaaaaaga
       61 aaaaggacaa gaagtacagc atcggcctgg ccatcggcac caactctgtg ggctgggccg
      121 tgatcaccga cgagtacaag gtgcccagca agaaattcaa ggtgctgggc aacaccgacc
      181 ggcacagcat caagaagaac ctgatcggag ccctgctgtt cgacagcggc gaaacagccg
      241 aggccacccg gctgaagaga accgccagaa gaagatacac cagacggaag aaccggatct
      301 gctatctgca agagatcttc agcaacgaga tggccaaggt ggacgacagc ttcttccaca
      361 gactggaaga gtccttcctg gtggaagagg ataagaagca cgagcggcac cccatcttcg
      421 gcaacatcgt ggacgaggtg gcctaccacg agaagtaccc caccatctac cacctgagaa
      481 agaaactggt ggacagcacc gacaaggccg acctgcggct gatctatctg gccctggccc
      541 acatgatcaa gttccggggc cacttcctga tcgagggcga cctgaacccc gacaacagcg
      601 acgtggacaa gctgttcatc cagctggtgc agacctacaa ccagctgttc gaggaaaacc
      661 ccatcaacgc cagcggcgtg gacgccaagg ccatcctgtc tgccagactg agcaagagca
      721 gacggctgga aaatctgatc gcccagctgc ccggcgagaa gaagaatggc ctgttcggca
      781 acctgattgc cctgagcctg ggcctgaccc ccaacttcaa gagcaacttc gacctggccg
      841 aggatgccaa actgcagctg agcaaggaca cctacga
//
