LOCUS       sequence-145681-         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified 
            scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of
            TFRC mRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Nelles DA, Fang MY, O'Connell MR, Xu JL, Markmiller SJ, Doudna JA, 
            Yeo GW
  TITLE     Programmable RNA Tracking in Live Cells with CRISPR/Cas9.
  JOURNAL   Cell. 2016 Mar 16. pii: S0092-8674(16)30204-5. doi: 
            10.1016/j.cell.2016.02.054.
  PUBMED    26997482
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2016 
            Mar 16. pii: S0092-8674(16)30204-5. doi: 
            10.1016/j.cell.2016.02.054."
COMMENT     Sequence Label: pBluescriptSKII+ U6-sgRNA(F+E) TFRC
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        85..333
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     85..105
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     256..275
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 ataagcttga tatcgtgtac aaaaaagcag gctttaaagg aaccaattca gtcgactgga
       61 tccggtacca aggtcgggca ggaagagggc ctatttccca tgattccttc atatttgcat
      121 atacgataca aggctgttag agagataatt agaattaatt tgactgtaaa cacaaagata
      181 ttagtacaaa atacgtgacg tanaaagtaa taatttcttg ggtagtttgc agttttaaaa
      241 ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg
      301 gctttatata tcttgtggaa aggacgaaac accgcattct tatctggtca gtgcgtttaa
      361 gagctatgct ggaaacagca tagcaagttt aaataaggct agtccgttat caacttgaaa
      421 aagtggcacc gagtcggtgc tttttttcct gcagcccggg ggatccagga tcaattccta
      481 ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag cggccccgct
      541 gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag
      601 gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt
      661 caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc
      721 acgtctcact agtctcgtgc a
//
