LOCUS       sequence-145684-         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Light-induced nuclear export switch fusion to nano for microscopy in
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Yumerefendi H, Lerner AM, Zimmerman SP, Hahn K, Bear JE, Strahl BD, 
            Kuhlman B
  TITLE     Light-induced nuclear export reveals rapid dynamics of epigenetic 
            modifications.
  JOURNAL   Nat Chem Biol. 2016 Jun;12(6):399-401. doi: 10.1038/nchembio.2068. 
            Epub 2016 Apr 18.
  PUBMED    27089030
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.2068"; journalName: "Nat Chem Biol"; date: 
            "2016-06"; volume: "12"; issue: "6"; pages: "399-401"
COMMENT     Sequence Label: pTriEx-mCherry-LINXa3-nano
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(43..66)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(73..105)
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             complement(148..477)
                     /codon_start=1
                     /product="SspB from Haemophilus influenzae, with the 
                     mutations Y11K and A15E to disrupt the natural dimerization
                     of the protein"
                     /label=SspB nano
                     /note="dimerizes with iLID at high affinity to create iLID 
                     nano (Guntas et al., 2015)"
                     /translation="SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQ
                     IVLNLSASATGNLQLTNDFIQFNARFKGVSRELYIPMGAALAIYARENGDGVMFEPEEI
                     YDELNI"
ORIGIN
        1 ccaccttctg ataggcagcc tgcacctgag gttaatcact tagtgatggt gatggtgatg
       61 gtggtgctcg agatcctcgg ggtcttccgg ggcgagttct ggctggcttg cacgtgtata
      121 cagctgtgcg gccgcaagct tttaaccaat attcagctcg tcatagattt cttctggttc
      181 gaacatcaca ccatcgccgt tctcgcgagc gtaaatggcc agagcggcac ccatcgggat
      241 atacagttca cgagacacgc ccttaaagcg ggcgttgaac tggataaaat catttgtcag
      301 ttgcaggttg ccggtcgcac ttgcagacag attcagcacg atctgaccgt ctttcacata
      361 ctccacgggc acgttcacgc ccaggtatgt ggcatccacc accagatatg gggtaaagct
      421 gttatcaacc agccaatcgt aatattcacg cagcagctta gggcgtttcg gggagctatg
      481 cattgatccg gaccccgacc ccagatccag cccggccagc tcttttgccg cttcatcaat
      541 attttctgca gttttcttaa tcagcatgac tccctctctc tcggcagcat ctcggacatg
      601 ctcagttcca tccaactgaa ccccaataaa gtactggaca tctcccttct gatctcgcat
      661 aggctgcaag tgaaagaggt tccagaactt tttaccactc tttgtataat taatcagctg
      721 aacagtgacc tctgtttggt tatctatggc atctctaatt tttctcactg tcgcgcgatc
      781 agtttcagga ccttgtagaa acctgcagtt tcttcccaaa atttcttcac ggctatattc
      841 tgtcaactgc aagaaactat cggacgcgaa tataatggga ttatctgg
//
