LOCUS       sequence-145685-         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Light-induced nuclear export switch fusion to nano for microscopy in
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Yumerefendi H, Lerner AM, Zimmerman SP, Hahn K, Bear JE, Strahl BD, 
            Kuhlman B
  TITLE     Light-induced nuclear export reveals rapid dynamics of epigenetic 
            modifications.
  JOURNAL   Nat Chem Biol. 2016 Jun;12(6):399-401. doi: 10.1038/nchembio.2068. 
            Epub 2016 Apr 18.
  PUBMED    27089030
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.2068"; journalName: "Nat Chem Biol"; date: 
            "2016-06"; volume: "12"; issue: "6"; pages: "399-401"
COMMENT     Sequence Label: pTriEx-mCherry-LINXa3-nano
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    19..43
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        59..168
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             183..890
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(328..346)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 aatacgactc actatagggg aattgtgagc ggataacaat tccccggagt taatccggga
       61 cctttaattc aacccaacac aatatattat agttaaataa gaattattat caaatcattt
      121 gtatattaat taaaatacta tactgtaaat tacattttat ttacaatcaa aggagatata
      181 ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca
      241 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
      301 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc
      361 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc
      421 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc
      481 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
      541 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccctg
      601 taatgcagaa gaagactatg ggctgggagg cctcctccga gcggatgtac cccgaggacg
      661 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg
      721 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg
      781 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
      841 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag ggtaccccgg
      901 cc
//
