LOCUS       sequence-145689-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Control of the E3 ligase Bre1 with LINXa in yeast..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Yumerefendi H, Lerner AM, Zimmerman SP, Hahn K, Bear JE, Strahl BD, 
            Kuhlman B
  TITLE     Light-induced nuclear export reveals rapid dynamics of epigenetic 
            modifications.
  JOURNAL   Nat Chem Biol. 2016 Jun;12(6):399-401. doi: 10.1038/nchembio.2068. 
            Epub 2016 Apr 18.
  PUBMED    27089030
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.2068"; journalName: "Nat Chem Biol"; date: 
            "2016-06"; volume: "12"; issue: "6"; pages: "399-401"
COMMENT     Sequence Label: pNIA-CEN-FLAG-LINXa4-Bre1
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             190..213
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             265..693
                     /codon_start=1
                     /gene="NPH1 from Avena sativa"
                     /product="light, oxygen, or voltage domain from phototropin
                     1 of Avena sativa (Herrou and Crosson, 2011)"
                     /label=LOV2
                     /note="photoswitch that undergoes a light-inducible 
                     conformational change"
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQ
                     YFIGVQLDGTEHVRDAAEREGVMLIKKTAENIDEAAKEL"
ORIGIN
        1 gtttgctgtc ttgctatcag tataaataga cctgcaatta ttaatctttt gtttcctcgt
       61 cattgttctc gttccctttc ttccttgttt ctttttctgc acaatatttc aagctatacc
      121 aagcatacaa tcaactccaa gcttgaatta attccgggcg gaatgaaagc gttaacgtct
      181 ggcggccgcg attacaagga cgacgatgac aagtctagag ccgccgccaa gagggtgaag
      241 ctggacggtt ctggaagtgg gtccttggct actacacttg aacgtattga gaagaacttt
      301 gtcattactg acccaagatt gccagataat cccattatat tcgcgtccga tagtttcttg
      361 cagttgacag aatatagccg tgaagaaatt ttgggaagaa actgcaggtt tctacaaggt
      421 cctgaaactg atcgcgcgac agtgagaaaa attagagatg ccatagataa ccaaacagag
      481 gtcactgttc agctgattaa ttatacaaag agtggtaaaa agttctggaa cctctttcac
      541 ttgcagccta tgcgagatca gaagggagat gtccagtact ttattggggt tcagttggat
      601 ggaactgagc atgtccgaga tgctgccgag agagagggag tcatgctgat taagaaaact
      661 gcagaaaata ttgatgaagc ggcaaaagag ctggccgggc tggatctggg gtctggaagt
      721 gggtcggggt cccggggaac ggccgagcct gctactgccg ctatcgcact agaattaagc
      781 gatcctagtg aaccgcttac tcaatctgat gtgatagcgt ttcaaaaaga agctttgttt
//
