LOCUS       sequence-145690-         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression vector encoding untagged WT human AMPK alpha 
            1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Toyama EQ, Herzig S, Courchet J, Lewis TL Jr, Loson OC, Hellberg K, 
            Young NP, Chen H, Polleux F, Chan DC, Shaw RJ
  TITLE     Metabolism. AMP-activated protein kinase mediates mitochondrial 
            fission in response to energy stress.
  JOURNAL   Science. 2016 Jan 15;351(6270):275-81. doi: 10.1126/science.aab4138.
  PUBMED    26816379
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2016-01-15- 15"; volume: "351"; 
            issue: "6270"; pages: "275-81"
COMMENT     Sequence Label: pLenti.CMV/TO_PRKAA1
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..38
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    79..103
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cctatcagtg atagagatct ccctatcagt gatagagatc gtcgactagt ccagtgtggt
       61 ggaattctgc agatatcaac aagtttgtac aaaaaagcag gcaccatggc gacagccgag
      121 aagcagaaac acgacgggcg ggtgaagatc ggccactaca ttctgggtga cacgctgggg
      181 gtcggcacct tcggcaaagt gaaggttggc aaacatgaat tgactgggca taaagtagct
      241 gtgaagatac tcaatcgaca gaagattcgg agccttgatg tggtaggaaa aatccgcaga
      301 gaaattcaga acctcaagct tttcaggcat cctcatataa ttaaactgta ccaggtcatc
      361 agtacaccat ctgatatttt catggtgatg gaatatgtct caggaggaga gctatttgat
      421 tatatctgta agaatggaag gctggatgaa aaagaaagtc ggcgtctgtt ccaacagatc
      481 ctttctggtg tggattattg tcacaggcat atggtggtcc atagagattt gaaacctgaa
      541 aatgtcctgc ttgatgcaca catgaatgca aagatagctg attttggtct ttcaaacatg
      601 atgtcagatg gtgaattttt aagaacaagt tgtggctcac ccaactatgc tgcaccagaa
      661 gtaatttcag gaagattgta tgcaggccca gaggtagata tatggagcag tggggttatt
      721 ctctatgctt tattatgtgg aacccttcca tttgatgatg accatgtgcc aactcttttt
      781 aagaagatat gtgatgggat cttctatacc cctcaatatt taaatccttc tgtgattagc
      841 cttttgaaac atatgctgca ggtggatccc
//
