LOCUS       sequence-145695-         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Visualizing the nuclear translocation of LINXa4-Bre1 in yeast..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Yumerefendi H, Lerner AM, Zimmerman SP, Hahn K, Bear JE, Strahl BD, 
            Kuhlman B
  TITLE     Light-induced nuclear export reveals rapid dynamics of epigenetic 
            modifications.
  JOURNAL   Nat Chem Biol. 2016 Jun;12(6):399-401. doi: 10.1038/nchembio.2068. 
            Epub 2016 Apr 18.
  PUBMED    27089030
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.2068"; journalName: "Nat Chem Biol"; date: 
            "2016-06"; volume: "12"; issue: "6"; pages: "399-401"
COMMENT     Sequence Label: pNIA-CEN-Venus-FLAG-LINXa4-Bre1
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(175..177,178..180,181..693)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(220..241)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(481..500)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     828..849
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tttgctgtct tgctatcaag tataaataga cctgcaatta ttaatctttt gtttcctcgt
       61 cattgttctc gttccctttc ttccttgttt ctttttctgc acaatatttc aagctatacc
      121 aagcatacaa tcaactccaa gcttgaatta attccgggcg gaatgaaagc gttcatggtg
      181 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
      241 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
      301 ctgaccctga agttgatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
      361 accaccctcg gctacggcct gcagtgcttc gcccgctacc ccgaccacat gaagcagcac
      421 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
      481 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
      541 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
      601 gagtacaact acaacagcca caacgtctat atcaccgccg acaagcagaa gaacggcatc
      661 aaggcaaact tcaagatccg ccacaacatc gaggacggcg gcgtgcagct cgccgaccac
      721 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
      781 agctaccagt ccaaactgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
      841 gagttcgtga ccgccgccgg gatcactctc ggcatggacg
//
