LOCUS       sequence-145697-       11435 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Visualizing the nuclear translocation of LINXa4-Bre1 in yeast..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11435)
  AUTHORS   Yumerefendi H, Lerner AM, Zimmerman SP, Hahn K, Bear JE, Strahl BD, 
            Kuhlman B
  TITLE     Light-induced nuclear export reveals rapid dynamics of epigenetic 
            modifications.
  JOURNAL   Nat Chem Biol. 2016 Jun;12(6):399-401. doi: 10.1038/nchembio.2068. 
            Epub 2016 Apr 18.
  PUBMED    27089030
REFERENCE   2  (bases 1 to 11435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.2068"; journalName: "Nat Chem Biol"; date: 
            "2016-06"; volume: "12"; issue: "6"; pages: "399-401"
COMMENT     Sequence Label: pNIA-CEN-Venus-FLAG-LINXa4-Bre1
FEATURES             Location/Qualifiers
     source          1..11435
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(88..93,94..111,112..118)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    126..142
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     131..153
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     150..166
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     150..166
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     185..205
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        187..205
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1007..1711
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             join(1750..1752,1753..1755,1756..2466)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1795..1816)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2056..2075)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2403..2424
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2500..2523
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2575..3003
                     /codon_start=1
                     /gene="NPH1 from Avena sativa"
                     /product="light, oxygen, or voltage domain from phototropin
                     1 of Avena sativa (Herrou and Crosson, 2011)"
                     /label=LOV2
                     /note="photoswitch that undergoes a light-inducible 
                     conformational change"
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQ
                     YFIGVQLDGTEHVRDAAEREGVMLIKKTAENIDEAAKEL"
     terminator      5167..5414
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(5183..5201)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(5432..5451)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5433..5451)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5461..5478)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5461..5477)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5470..5492)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5613..5630)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5784..6372)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5864..5883)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6543..7334,7335..7403))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7166..7185
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7404..7508)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7576..7594
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7614..8018
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             8031..9125
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     misc_feature    9487..10649
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN4 centromere fused to the 
                     autonomously replicating sequence ARS1/ARS416"
     rep_origin      9487..10324
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1/ARS416"
ORIGIN
        1 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
       61 ttacctcact cattaggcac cccaggcttt acactttatg cttccggctc ctatgttgtg
      121 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
      181 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctctaaaaca agaagagggt
      241 tgactacatc acgatgaggg ggatcgaaga aatgatggta aatgaaatag gaaatcaagg
      301 agcatgaagg caaaagacaa atataagggt cgaacgaaaa ataaagtgaa aagtgttgat
      361 atgatgtatt tggctttgcg gcgccgaaaa aacgagttta cgcaattgca caatcatgct
      421 gactctgtgg cggacccgcg ctcttgccgg cccggcgata acgctgggcg tgaggctgtg
      481 cccggcggag ttttttgcgc ctgcattttc caaggtttac cctgcgctaa ggggcgagat
      541 tggagaagca ataagaatgc cggttggggt tgcgatgatg acgaccacga caactggtgt
      601 cattatttaa gttgccgaaa gaacctgagt gcatttgcaa catgagtata ctagaagaat
      661 gagccaagac ttgcgagacg cgagtttgcc ggtggtgcga acaatagagc gaccatgacc
      721 ttgaaggtga gacgcgcata accgctagag tactttgaag aggaaacagc aatagggttg
      781 ctaccagtat aaatagacag gtacatacaa cactggaaat ggttgtctgt ttgagtacgc
      841 tttcaattca tttgggtgtg cactttatta tgttacaata tggaagggaa ctttacactt
      901 ctcctatgca catatattaa ttaaagtcca atgctagtag agaagggggg taacacccct
      961 ccgcgctctt ttccgatttt tttctaaacc gtggaatatt tcggatatcc ttttgttgtt
     1021 tccgggtgta caatatggac ttcctctttt ctggcaacca aacccataca tcgggattcc
     1081 tataatacct tcgttggtct ccctaacatg taggtggcgg aggggagata tacaatagaa
     1141 cagataccag acaagacata atgggctaaa caagactaca ccaattacac tgcctcattg
     1201 atggtggtac ataacgaact aatactgtag ccctagactt gatagccatc atcatatcga
     1261 agtttcacta ccctttttcc atttgccatc tattgaagta ataataggcg catgcaactt
     1321 cttttctttt tttttctttt ctctctcccc cgttgttgtc tcaccatatc cgcaatgaca
     1381 aaaaaaatga tggaagacac taaaggaaaa aattaacgac aaagacagca ccaacagatg
     1441 tcgttgttcc agagctgatg aggggtatct tcgaacacac gaaacttttt ccttccttca
     1501 ttcacgcaca ctactctcta atgagcaacg gtatacggcc ttccttccag ttacttgaat
     1561 ttgaaataaa aaaagtttgc tgtcttgcta tcaagtataa atagacctgc aattattaat
     1621 cttttgtttc ctcgtcattg ttctcgttcc ctttcttcct tgtttctttt tctgcacaat
     1681 atttcaagct ataccaagca tacaatcaac tccaagcttg aattaattcc gggcggaatg
     1741 aaagcgttca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     1801 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     1861 gccacctacg gcaagctgac cctgaagttg atctgcacca ccggcaagct gcccgtgccc
     1921 tggcccaccc tcgtgaccac cctcggctac ggcctgcagt gcttcgcccg ctaccccgac
     1981 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     2041 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     2101 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     2161 ctggggcaca agctggagta caactacaac agccacaacg tctatatcac cgccgacaag
     2221 cagaagaacg gcatcaaggc aaacttcaag atccgccaca acatcgagga cggcggcgtg
     2281 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     2341 gacaaccact acctgagcta ccagtccaaa ctgagcaaag accccaacga gaagcgcgat
     2401 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     2461 tacaagggaa gtagcggttc tagaacgtct ggcggccgcg attacaagga cgacgatgac
     2521 aagtctagag ccgccgccaa gagggtgaag ctggacggtt ctggaagtgg gtccttggct
     2581 actacacttg aacgtattga gaagaacttt gtcattactg acccaagatt gccagataat
     2641 cccattatat tcgcgtccga tagtttcttg cagttgacag aatatagccg tgaagaaatt
     2701 ttgggaagaa actgcaggtt tctacaaggt cctgaaactg atcgcgcgac agtgagaaaa
     2761 attagagatg ccatagataa ccaaacagag gtcactgttc agctgattaa ttatacaaag
     2821 agtggtaaaa agttctggaa cctctttcac ttgcagccta tgcgagatca gaagggagat
     2881 gtccagtact ttattggggt tcagttggat ggaactgagc atgtccgaga tgctgccgag
     2941 agagagggag tcatgctgat taagaaaact gcagaaaata ttgatgaagc ggcaaaagag
     3001 ctggccgggc tggatctggg gtctggaagt gggtcggggt cccggggaac ggccgagcct
     3061 gctactgccg ctatcgcact agaattaagc gatcctagtg aaccgcttac tcaatctgat
     3121 gtgatagcgt ttcaaaaaga agctttgttt agatgtataa atcgtcgaag agtcgacttt
     3181 gaagccttgc gaaagcaata tgaactttca agaagggagt gcatagatgt ttcccgcaag
     3241 ttggcaaata ttatggcgct gatagtaact ttagctaggt ttatcgaaac attctgcaca
     3301 gacgccaacg agaagcaact atgtagggaa atagcccaag gcgatgagac gttgatagtt
     3361 cagcgcagtg attctttcat gaagctactg actaaatatg ggaagccaaa cacgacagat
     3421 agtaatacta atagtaatgc ttcggatcat attcaagaat taacaacgga gttaaaaaac
     3481 ttgcggaaaa gtaaagagga gctcttttat gaaaattcgc aattgacaga ggagatttct
     3541 gctttgaagg aatattatac aaatataatt cgcaagtatg atagagatga atcctttact
     3601 ataaaacgtg tttttaagga agataagact gatgctgtaa aggaattacg ggaagatgaa
     3661 aaagaatcta atgaaaacaa tataaaatct ggaaataaag actcctctgc aattaatggt
     3721 gataatacat ccaaaaaatc cgagaaaggt gatgaacttg tgcaggcgga ggatgaacga
     3781 aaagaagatg ctgagaatga aaaattagaa ttggatctga aattttctga tttacgggca
     3841 gagataaact ccttgagttc cactataaaa gaccttgaaa atatcagaag ggaaaacgag
     3901 gaagagctca tcaaaacacg ttctgaggta tccaatttaa aaaagcaaca gatagcagca
     3961 gcagaccaag acccagattt caagtcatat gaccatgagt ccctgttagc aaaaattcaa
     4021 catttgactg aacaaaacgc agaattatct gagatcaata gttccttttt atcaaaattt
     4081 caagttttag ccaaagagaa agaaatatat acgaagaagg tccgagaaga attccagaaa
     4141 agtctcgact ccctagtaga aatgaacagt tcattggaaa aggacgttgt aagaataaga
     4201 acggctcgtg atgatttact aagcaaaatc gccattttgg aagctgaaaa atccaagact
     4261 gaagtgttga gcgatttaca acatgcaatt gatattttga agaagcaatg gacaaaaatt
     4321 gatcaaagat caaacgatac aaaatcctcg tcgactcaag atgcattaat aaaggaaatt
     4381 caagacttag agaagggttt tagagagtta tccgacttaa ctcataaaaa gtactctgag
     4441 attattaatc acgaatctgt tatatccaag cttaccgtag aaaaaactaa ggctgatcag
     4501 aaatattttg ccgcgatgag gtccaaagat tcaattttaa ttgaaatcaa aactctttct
     4561 aaaagcctca gtaaatcaaa tgagcttatt ctacagttaa aagactctga tagattgcta
     4621 cagcaaaaaa taggaaattt acataaacaa ctggatcttt ctcaaaataa tgaacgcagg
     4681 ttaattgatt ccagtaaaac agagacacta aaaataattg atttaaataa tacatccact
     4741 aaactgaaaa ggtcattaga aaagctacaa gaagaaagta acaaaagcat tgcagatatg
     4801 acgcatctag aaactaaatt aaatgataca gaaatagaat tgaagcactt caagcagaag
     4861 gcatctcatc tagaatccaa gtgtgaaaaa ctacatgata cattgttccg tggtaacaac
     4921 aaaaataagg gctcgagcga tgaagcactc gtagaagaac tagcaaattt ccgtacactt
     4981 gtttattgtt ccctgtgttc taaaaattgg aaaaacatgg ctattaaaac ctgtggccat
     5041 gtcttttgtg agaattgctg taaggaacga ttggcagcga gaatgagaaa atgccctaca
     5101 tgcaacaaag cattcagttc caatgattta ttgacagtgc acttgtaagg atccgtcgac
     5161 ctcgagtcat gtaattagtt atgtcacgct tacattcacg ccctcccccc acatccgctc
     5221 taaccgaaaa ggaaggagtt agacaacctg aagtctaggt ccctatttat ttttttatag
     5281 ttatgttagt attaagaacg ttatttatat ttcaaatttt tctttttttt ctgtacaaac
     5341 gcgtgtacgc atgtaacatt atactgaaaa ccttgcttga gaaggttttg ggacgctcga
     5401 aggctttaat ttgcggccgg tacccaattc gccctatagt gagtcgtatt acgcgcgctc
     5461 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     5521 ccttgcagca catccccctt tcgccagctg cattaatgaa tcggccaacg cgcggggaga
     5581 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     5641 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     5701 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     5761 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     5821 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     5881 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     5941 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     6001 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     6061 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     6121 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     6181 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     6241 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     6301 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     6361 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     6421 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     6481 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     6541 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     6601 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     6661 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     6721 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     6781 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     6841 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     6901 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     6961 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     7021 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     7081 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     7141 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     7201 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     7261 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     7321 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     7381 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     7441 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     7501 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     7561 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attaactgtg
     7621 ggaatactca ggtatcgtaa gatgcaagag ttcgaatctc ttagcaacca ttattttttt
     7681 cctcaacata acgagaacac acaggggcgc tatcgcacag aatcaaattc gatgactgga
     7741 aattttttgt taatttcaga ggtcgcctga cgcatatacc tttttcaact gaaaaattgg
     7801 gagaaaaagg aaaggtgaga ggccggaacc ggcttttcat atagaataga gaagcgttca
     7861 tgactaaatg cttgcatcac aatacttgaa gttgacaata ttatttaagg acctattgtt
     7921 ttttccaata ggtggttagc aatcgtctta ctttctaact tttcttacct tttacatttc
     7981 agcaatatat atatatattt caaggatata ccattctaat gtctgcccct atgtctgccc
     8041 ctaagaagat cgtcgttttg ccaggtgacc acgttggtca agaaatcaca gccgaagcca
     8101 ttaaggttct taaagctatt tctgatgttc gttccaatgt caagttcgat ttcgaaaatc
     8161 atttaattgg tggtgctgct atcgatgcta caggtgtccc acttccagat gaggcgctgg
     8221 aagcctccaa gaaggttgat gccgttttgt taggtgctgt gggtggtcct aaatggggta
     8281 caggtagtgt tagacctgaa caaggtttac taaaaatccg taaagaactt caattgtacg
     8341 ccaacttaag accatgtaac tttgcatccg actctctttt agacttatct ccaatcaagc
     8401 cacaatttgc taaaggtact gacttcgttg ttgtcagaga attagtggga ggtatttact
     8461 ttggtaagag aaaggaagac gatggtgatg gtgtcgcttg ggatagtgaa caatacaccg
     8521 ttccagaagt gcaaagaatc acaagaatgg ccgctttcat ggccctacaa catgagccac
     8581 cattgcctat ttggtccttg gataaagcta atgttttggc ctcttcaaga ttatggagaa
     8641 aaactgtgga ggaaaccatc aagaacgaat ttcctacatt gaaggttcaa catcaattga
     8701 ttgattctgc cgccatgatc ctagttaaga acccaaccca cctaaatggt attataatca
     8761 ccagcaacat gtttggtgat atcatctccg atgaagcctc cgttatccca ggttccttgg
     8821 gtttgttgcc atctgcgtcc ttggcctctt tgccagacaa gaacaccgca tttggtttgt
     8881 acgaaccatg ccacggttct gctccagatt tgccaaagaa taaggttgac cctatcgcca
     8941 ctatcttgtc tgctgcaatg atgttgaaat tgtcattgaa cttgcctgaa gaaggtaagg
     9001 ccattgaaga tgcagttaaa aaggttttgg atgcaggtat cagaactggt gatttaggtg
     9061 gttccaacag taccaccgaa gtcggtgatg ctgtcgccga agaagttaag aaaatccttg
     9121 cttaaaaaga ttctcttttt ttatgatatt tgtacataaa ctttataaat gaaattcata
     9181 atagaaacga cacgaaatta caaaatggaa tatgttcata gggtagaatt aattctcatg
     9241 tttgacagct tatcatcgat cgtccaactg catggagatg agtcgtggca agaataccaa
     9301 gagttcctcg gtttgccagt tattaaaaga ctcgtatttc caaaagactg caacatacta
     9361 ctcagtgcag cttcacagaa acctcattcg tttattccct tgtttgattc agaagcaggt
     9421 gggacaggtg aacttttgga ttggaactcg atttctgact gggttggaag gcaagagagc
     9481 cccgagagct tacattttat gttagctggt ggactgacgc cagaaaatgt tggtgatgcg
     9541 cttagattaa atggcgttat tggtgttgat gtaagcggag gtgtggagac aaatggtgta
     9601 aaagactcta acaaaatagc aaatttcgtc aaaaatgcta agaaataggt tattactgag
     9661 tagtatttat ttaagtattg tttgtgcact tgcctgcaag ccttttgaaa agcaagcata
     9721 aaagatctaa acataaaatc tgtaaaataa caagatgtaa agataatgct aaatcatttg
     9781 gctttttgat tgattgtaca ggaaaatata catcgcaggg ggttgacttt taccatttca
     9841 ccgcaatgga atcaaacttg ttgaagagaa tgttcacagg cgcatacgct acaatgaccc
     9901 gattcttgct agccttttct cggtcttgca aacaaccgcc ggcagcttag tatataaata
     9961 cacatgtaca tacctctctc cgtatcctcg taatcatttt cttgtattta tcgtcttttc
    10021 gctgtaaaaa ctttatcaca cttatctcaa atacacttat taaccgcttt tactattatc
    10081 ttctacgctg acagtaatat caaacagtga cacatattaa acacagtggt ttctttgcat
    10141 aaacaccatc agcctcaagt cgtcaagtaa agatttcgtg ttcatgcaga tagataacaa
    10201 tctatatgtt gataattagc gttgcctcat caatgcgaga tccgtttaac cggaccctag
    10261 tgcacttacc ccacgttcgg tccactgtgt gccgaacatg ctccttcact attttaacat
    10321 gtggaattaa ttctcatgtt tgacagctta tcatcgaact ctaagaggtg atacttattt
    10381 actgtaaaac tgtgacgata aaaccggaag gaagaataag aaaactcgaa ctgatctata
    10441 atgcctattt tctgtaaaga gtttaagcta tgaaagcctc ggcattttgg ccgctcctag
    10501 gtagtgcttt ttttccaagg acaaaacagt ttctttttct tgagcaggtt ttatgtttcg
    10561 gtaatcataa acaataaata aattatttca tttatgttta aaaataaaaa ataaaaaagt
    10621 attttaaatt tttaaaaaag ttgattataa gcatgtgacc ttttgcaagc aattaaattt
    10681 tgcaatttgt gattttaggc aaaagttaca atttctggct cgtgtaatat atgtatgcta
    10741 aagtgaactt ttacaaagtc gatatggact tagtcaaaag aaattttctt aaaaatatat
    10801 agcactagcc aatttagcac ttctttatga gatatattat agactttatt aagccagatt
    10861 tgtgtattat atgtatttac ccggcgaatc atggacatac attctgaaat aggtaatatt
    10921 ctctatggtg agacagcata gataacctag gatacaagtt aaaagctagt actgttttgc
    10981 agtaattttt ttctttttta taagaatgtt accacctaaa taagttataa agtcaatagt
    11041 taagtttgat atttgattgt aaaataccgt aatatatttg catgatcaaa aggctcaatg
    11101 ttgactagcc agcatgtcaa ccactatatt gatcaccgat atatggactt ccacaccaac
    11161 tagtaatatg acaataaatt caagatattc ttcatgagaa tggcccagcg atatatgcgg
    11221 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc
    11281 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccaggga
    11341 tacaagttaa aagctagtac tgttttgcag taattttttt cttttttata agaatgttac
    11401 cacctaaata agttataaag tcaatagtta agttt
//
