LOCUS       sequence-145698-         671 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  template for C-terminal TEV-site + 2x V5-tag (short version), 
            Blasticidin-Resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Kunzelmann S, Bottcher R, Schmidts I, Forstemann K
  TITLE     A Comprehensive Toolbox for Genome Editing in Cultured Drosophila 
            melanogaster Cells.
  JOURNAL   G3 (Bethesda). 2016 Jun 1;6(6):1777-85. doi: 10.1534/g3.116.028241.
  PUBMED    27172193
REFERENCE   2  (bases 1 to 671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.116"; 
            journalName: "G3 (Bethesda)"; date: "2016-06-1- 1"; volume: "6"; 
            issue: "6"; pages: "1777-85"
COMMENT     Sequence Label: pKF296
FEATURES             Location/Qualifiers
     source          1..671
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    237..270
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             complement(367..387)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(421..440)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(422..440)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tttatgtttg agttttatga tttatatttt aagttatttc aactgcaaca ccagcaccac
       61 gacctactca cagcaaaaaa cgtacaagaa ggaaagaagg aataaaaaga gtggtattct
      121 cttacaatat gttttatggc ataaaaggtg tggccattca tatcaaatat aaagtagtgt
      181 tgtttaacgt tatttttgta ggttgaatag tatattccaa cagcatatgt ttatttgaag
      241 ttcctattct ctagaaagta taggaacttc actagttcag ctgccatcca gacccagcag
      301 tgggttgggg atgctaggag ctgctgggct atccaggccc aacagggggt ttgggatgct
      361 ggcaccgccc tggaagtaca ggttctcctc gagccatccg gaagatctgg cggccgctct
      421 ccctatagtg agtcgtatta cgccggatgg atatggtgtt caggcacaag tgttaaagca
      481 gttgatttta ttcactatga tgaaaaaaac aatgaatgga acctgctcca agttaaaaat
      541 agagataata ccgaaaactc atcgagtagt aagattagag ataatacaac aataaaaaaa
      601 tggtttagaa cttactcaca gcgtgatgct actaattggg acaattttcc agatgaagta
      661 tcatctaaga a
//
