LOCUS       sequence-145699-         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  template for C-terminal TEV-site + 2x V5-tag (short version), 
            Blasticidin-Resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Kunzelmann S, Bottcher R, Schmidts I, Forstemann K
  TITLE     A Comprehensive Toolbox for Genome Editing in Cultured Drosophila 
            melanogaster Cells.
  JOURNAL   G3 (Bethesda). 2016 Jun 1;6(6):1777-85. doi: 10.1534/g3.116.028241.
  PUBMED    27172193
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.116"; 
            journalName: "G3 (Bethesda)"; date: "2016-06-1- 1"; volume: "6"; 
            issue: "6"; pages: "1777-85"
COMMENT     Sequence Label: pKF296
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(109..142)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 tacttccagg gcggtgccag catcccaaac cccctgttgg gcctggatag cccagcagct
       61 cctagcatcc ccaacccact gctgggtctg gatggcagct gaactagtga agttcctata
      121 ctttctagag aataggaact tcaaataaac atatgctgtt ggaatatact attcaaccta
      181 caaaaataac gttaaacaac actactttat atttgatatg aatggccaca ccttttatgc
      241 cataaaacat attgtaagag aataccactc tttttattcc ttctttcctt cttgtacgtt
      301 ttttgctgtg agtaggtcgt ggtgctggtg ttgcagttga aataacttaa aatataaatc
      361 ataaaactca aacataaact tgactattta tttatttatt aagaaaggag gtaccggtat
      421 ggccaagcct ttgtctcaag aagaatccac cctcattgaa agagcaacgg ctacaatcaa
      481 cagcatcccc atctctgaag actacagcgt cgccagcgca gctctctcta gcgacggccg
      541 catcttcact ggtgtcaatg tatatcattt tactggggga ccttgcgcag aactcgtggt
      601 gctgggcact gctgctgctg cggcagctgg caacctgact tgtatcgtcg cgatcggaaa
      661 tgagaacagg ggcatcttga gcccctgcgg acggtgccga caggttcttc tcgatctgca
      721 tcctgggatc aaagccatag tgaaggacag tgatggacag ccgacggcag ttgg
//
