LOCUS       sequence-145700-         676 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  template for C-terminal TEV-site + 2x V5-tag (short version), 
            Puromycin-Resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Kunzelmann S, Bottcher R, Schmidts I, Forstemann K
  TITLE     A Comprehensive Toolbox for Genome Editing in Cultured Drosophila 
            melanogaster Cells.
  JOURNAL   G3 (Bethesda). 2016 Jun 1;6(6):1777-85. doi: 10.1534/g3.116.028241.
  PUBMED    27172193
REFERENCE   2  (bases 1 to 676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.116"; 
            journalName: "G3 (Bethesda)"; date: "2016-06-1- 1"; volume: "6"; 
            issue: "6"; pages: "1777-85"
COMMENT     Sequence Label: pKF297
FEATURES             Location/Qualifiers
     source          1..676
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(115..148)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 aacctgtact tccagggcgg tgccagcatc ccaaaccccc tgttgggcct ggatagccca
       61 gcagctccta gcatccccaa cccactgctg ggtctggatg gcagctgaac tagtgaagtt
      121 cctatacttt ctagagaata ggaacttcaa ataaacatat gggcgcgccc tgttggaata
      181 tactattcaa cctacaaaaa taacgttaaa caacactact ttatatttga tatgaatggc
      241 cacacctttt atgccataaa acatattgta agagaatacc actcttttta ttccttcttt
      301 ccttcttgta cgttttttgc tgtgagtagg tcgtggtgct ggtgttgcag ttgaaataac
      361 ttaaaatata aatcataaaa ctcaaacata aacttgacta tttatttatt tattaagaaa
      421 ggaggtaccg gtggcgcgcc atgaccgagt acaagcctac agtgcgactg gccacacgcg
      481 atgacgttcc cagagccgtt cggacgttgg ctgccgcgtt tgcggactat cccgcaactc
      541 gccatactgt ggaccctgat cgccacatcg agagggtgac tgagcttcag gagctgttcc
      601 tgaccagggt agggctggac ataggcaagg tctgggtggc cgacgatgga gccgctgttg
      661 cggtgtggac tacccc
//
