LOCUS       sequence-145701-         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  template for C-terminal TEV-site + 2x V5-tag (short version), 
            Puromycin-Resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Kunzelmann S, Bottcher R, Schmidts I, Forstemann K
  TITLE     A Comprehensive Toolbox for Genome Editing in Cultured Drosophila 
            melanogaster Cells.
  JOURNAL   G3 (Bethesda). 2016 Jun 1;6(6):1777-85. doi: 10.1534/g3.116.028241.
  PUBMED    27172193
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.116"; 
            journalName: "G3 (Bethesda)"; date: "2016-06-1- 1"; volume: "6"; 
            issue: "6"; pages: "1777-85"
COMMENT     Sequence Label: pKF297
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    264..297
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             complement(394..414)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(448..467)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(449..467)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(773..791)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ataataataa atagtcaagt ttatgtttga gttttatgat ttatatttta agttatttca
       61 actgcaacac cagcaccacg acctactcac agcaaaaaac gtacaagaag gaaagaagga
      121 ataaaaagag tggtattctc ttacaatatg ttttatggca taaaaggtgt ggccattcat
      181 atcaaatata aagtagtgtt gtttaacgtt atttttgtag gttgaatagt atattccaac
      241 agggcgcgcc catatgttta tttgaagttc ctattctcta gaaagtatag gaacttcact
      301 agttcagctg ccatccagac ccagcagtgg gttggggatg ctaggagctg ctgggctatc
      361 caggcccaac agggggtttg ggatgctggc accgccctgg aagtacaggt tctcctcgag
      421 ccatccggaa gatctggcgg ccgctctccc tatagtgagt cgtattacgc cggatggata
      481 tggtgttcag gcacaagtgt taaagcagtt gattttattc actatgatga aaaaaacaat
      541 gaatggaacc tgctccaagt taaaaataga gataataccg aaaactcatc gagtagtaag
      601 attagagata atacaacaat aaaaaaatgg tttagaactt actcacagcg tgatgctact
      661 aattgggaca attttccaga tgaagtatca tctaagaatt taaatgaaga agacttcaga
      721 gcttttgtta aaaattattt ggcaaaaata atataattcg gctgcagggg cggcctcgtg
      781 atacgcctat tttt
//
